Soft X-ray Contact Microscopy (SXCM) experiments have been performed on the nematode Caenorhabditis elegans using the Prague Asterix Iodine Laser System (PALS). Pulsed X-rays were generated using gold and molybdenum targets with laser intensities I ≥ 1014 W/cm2 to record the contact images of C. elegans, on PMMA photoresists. Results were analyzed using an atomic force microscope operating in constant force mode. Our results show some characteristic features of the C. elegans and the suitability of the SXCM technique for the study of multi-cellular specimens.
Investigation of Caenorhabditis elegans using Soft X ray Contact Microscopy / T. Desai, D. Batani, A. Bernardinello, G. Poletti, F. Orsini, J. Ullschmied, J. Skala, B. Kralikova, E. Krousky, M. Pfeifer, C. Kadlec, T. Mocek, A.Prag, O. Renner, L. Juha, F. Cotelli, C. Lora Lamia, A. Zullini. - In: PHYSICA MEDICA. - ISSN 1120-1797. - 20:3(2004), pp. 121-126.
Investigation of Caenorhabditis elegans using Soft X ray Contact Microscopy
F. Orsini;F. Cotelli;C. Lora Lamia;
2004
Abstract
Soft X-ray Contact Microscopy (SXCM) experiments have been performed on the nematode Caenorhabditis elegans using the Prague Asterix Iodine Laser System (PALS). Pulsed X-rays were generated using gold and molybdenum targets with laser intensities I ≥ 1014 W/cm2 to record the contact images of C. elegans, on PMMA photoresists. Results were analyzed using an atomic force microscope operating in constant force mode. Our results show some characteristic features of the C. elegans and the suitability of the SXCM technique for the study of multi-cellular specimens.
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