A case of fatal intoxication from metformin is presented. The decedent was an obese 58-year-old-woman with type II diabetes, in whom severe lactic acidosis secondary to metformin accumulation was precipitated by acute renal failure. She had been on metformin 500 mg twice a day. Postmortem blood was deproteinated with acetonitrile, washed with dichloromethane, and the resulting supernatant injected into high-performance liquid chromatography system. Separation was performed on a analytical 125 × 4 mm i.d. RP-8 column. The wavelength was set at 235 nm. The mobile phase was acetonitrile (40%), sodium lauryl sulfate, and sodium dihydrogen phosphate adjusted to pH 5.1 (60%) at a flow rate of 1.0 mL/min. The concentration of metformin in postmortem blood was 77.3 μg/mL. The qualitative result was also confirmed by LC/APCI/MS/MS analysis.
A case of fatal intoxication from metformin / V. Gambaro, L. Dell'Acqua, F. Farè, M. Fidani, R. Froldi, E. Saligari. - In: JOURNAL OF FORENSIC SCIENCES. - ISSN 0022-1198. - 52:4(2007), pp. 988-991.
|Titolo:||A case of fatal intoxication from metformin|
GAMBARO, VENIERO (Primo)
DELL'ACQUA, LUCIA SPERANZA MARIA (Secondo)
|Settore Scientifico Disciplinare:||Settore CHIM/08 - Chimica Farmaceutica|
Settore MED/43 - Medicina Legale
|Data di pubblicazione:||2007|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1111/j.1556-4029.2007.00461.x|
|Appare nelle tipologie:||01 - Articolo su periodico|