n this paper, the possible involvement of the acute phase protein alpha(1)-acid glycoprotein (AGP) in the local immunomodulation of inflammation was investigated. The dose response of bovine neutrophils to AGP as to mobilization of primary and secondary granules was studied. It was found that AGP fulfils a protective role against spontaneous exocytosis of secondary, but not primary, granules. This downregulatory effect is much more evident when degranulation is challenged with Zymosan activated serum (ZAS). AGP activity is dose-dependent, the acute phase concentration being more active than the physiological one. Carbohydrate moiety of AGP was found to be critical, since experimentally desialylated protein does not maintain its exocytosis-modulatory activity. The fact that AGP may modulate the degranulation of neutrophils confirms the hypothesis that AGP is heavily involved in the fine tuning of neutrophil activity in the inflammatory environment.

Down-regulatory effect of alpha(1)-acid glycoprotein on bovine neutrophil degranulation / A. Miranda-Ribera, C. Lecchi, V. Bronzo, L.M. Scaccabarozzi, P. Sartorelli, F. Franciosi, F. Ceciliani. - In: COMPARATIVE IMMUNOLOGY, MICROBIOLOGY AND INFECTIOUS DISEASES. - ISSN 0147-9571. - 33:4(2010 Jul), pp. 291-306. [10.1016/j.cimid.2008.10.009]

Down-regulatory effect of alpha(1)-acid glycoprotein on bovine neutrophil degranulation

A. Miranda-Ribera
Primo
;
C. Lecchi
Secondo
;
V. Bronzo;L.M. Scaccabarozzi;P. Sartorelli;F. Franciosi
Penultimo
;
F. Ceciliani
Ultimo
2010

Abstract

n this paper, the possible involvement of the acute phase protein alpha(1)-acid glycoprotein (AGP) in the local immunomodulation of inflammation was investigated. The dose response of bovine neutrophils to AGP as to mobilization of primary and secondary granules was studied. It was found that AGP fulfils a protective role against spontaneous exocytosis of secondary, but not primary, granules. This downregulatory effect is much more evident when degranulation is challenged with Zymosan activated serum (ZAS). AGP activity is dose-dependent, the acute phase concentration being more active than the physiological one. Carbohydrate moiety of AGP was found to be critical, since experimentally desialylated protein does not maintain its exocytosis-modulatory activity. The fact that AGP may modulate the degranulation of neutrophils confirms the hypothesis that AGP is heavily involved in the fine tuning of neutrophil activity in the inflammatory environment.
α1-Acid glycoprotein; Acute phase reaction; Exocytosis; Granulocytes
Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/144345
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