The in vitro efficacy of ceftriaxone plus amikacin combination against gram-positive and gram-negative bacteria, clinically isolated from patients affected by pneumonia in intensive care units, was compared to that of the 2 drugs used alone. The study was performed using a dynamic model in which the human kinetics of the drugs after intramuscular administration was simulated. The antibacterial activity was tested by determining the bacterial cell count (CFU/ml). Killing curves came out from plotting the log CFU/ml versus time. In the same way, ceftriaxone and amikacin concentrations were assayed by HPLC and fluorescence polarization immunoassay, respectively. The results show that ceftriaxone plus amikacin combination exert a high killing activity against all tested strains. The two antibiotics alone initially have a good killing activity but this is followed by bacterial regrowth for all tested isolates. This data supports the results of several clinical studies which have shown a good therapeutic efficacy of ceftriaxone plus amikacin combination in the treatment of severe infections caused by organisms intermediately sensitive to these drugs.
Bactericidal kinetics of an in vitro infection model of once-daily ceftriaxone plus amikacin against gram-positive and gram-negative bacteria / F. Scaglione, S. Dugnani, G. Demartini, M.M.Arcidiacono, C. Cocuzza, F. Fraschini. - In: CHEMOTHERAPY. - ISSN 0009-3157. - 41:4(1995), pp. 239-246.
|Titolo:||Bactericidal kinetics of an in vitro infection model of once-daily ceftriaxone plus amikacin against gram-positive and gram-negative bacteria|
SCAGLIONE, FRANCESCO (Primo)
DUGNANI, SILVANA (Secondo)
FRASCHINI, FRANCO LUIGI (Ultimo)
|Parole Chiave:||Amikacin; Ceftriaxone; Pharmacokinetics|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||1995|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1159/000239351|
|Appare nelle tipologie:||01 - Articolo su periodico|