An in vitro infection model was created using a suspension of macrophages, polymorphonuclear leukocytes, lymphocytes, fibroblasts, and human serum to which pathogen and antibiotic were added. Separate intracellular and extracellular antibiotic concentrations and activity against Staphylococcus aureus and Legionella pneumophila were assessed for three antimicrobial agents: amoxicillin, azithromycin and clarithromycin. Amoxicillin was found almost exclusively in extracellular fluid, where it was active; intracellularly, it was ineffective. Azithromycin, in contrast, was primarily concentrated and active intracellularly, with little activity in extracellular fluid. Clarithromycin was present in both compartments and possessed significant activity both intracellularly and extracellularly.

A new model examining intracellular and extracellular activity of amoxicillin, azithromycin, and clarithromycin in infected cells / F. Scaglione, G. Demartini, S. Dugnani, F. Fraschini. - In: CHEMOTHERAPY. - ISSN 0009-3157. - 39:6(1993), pp. 416-423.

A new model examining intracellular and extracellular activity of amoxicillin, azithromycin, and clarithromycin in infected cells

F. Scaglione
Primo
;
G. Demartini
Secondo
;
S. Dugnani
Penultimo
;
F. Fraschini
Ultimo
1993

Abstract

An in vitro infection model was created using a suspension of macrophages, polymorphonuclear leukocytes, lymphocytes, fibroblasts, and human serum to which pathogen and antibiotic were added. Separate intracellular and extracellular antibiotic concentrations and activity against Staphylococcus aureus and Legionella pneumophila were assessed for three antimicrobial agents: amoxicillin, azithromycin and clarithromycin. Amoxicillin was found almost exclusively in extracellular fluid, where it was active; intracellularly, it was ineffective. Azithromycin, in contrast, was primarily concentrated and active intracellularly, with little activity in extracellular fluid. Clarithromycin was present in both compartments and possessed significant activity both intracellularly and extracellularly.
Amoxicillin; Antibiotic intracellular activity; Azithromycin; Clarithromycin; Killing rate
Settore BIO/14 - Farmacologia
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/141077
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