In vivo studies suggest that the phenolic component contributes to the anti-inflammatory and antiatherosclerotic actions of olive oil; however, the effects In circulating cells are not fully characterized. Monocytes play a key role in inflammation-based diseases by expressing several molecules, Including metalloproteinases (MMPs). In the present study, we investigated the effects of olive oil phenolic extract and Individual compounds on MMP-9 in THP-1 cells, a human monocyte-like cell line. Olive oil extract prevented the stimulation of MMP-9 expression and secretion in tumor necrosis factor α-treated THP-1 cells. Oleuropein aglycone, a typical olive oil phenol, was active at concentrations found in the extract, although other compounds probably contribute to the biological activity. We also found that the effect of the extract and individual compounds on MMP-9 is due to impaired nuclear factor-kB signaling. Our findings provide further evidence on the mechanisms by which olive oil reduces the inflammatory burden associated with disorders, such as atherosclerosis.

Olive Oil phenols modulate the expression of metalloproteinase 9 in THP-1 cells by acting on nuclear factor-kB signaling / M. Dell'Agli, R. Fagnani, G. Galli, O. Maschi, F. Gilardi, S. Bellosta, M. Crestani, E. Bosisio, E. De Fabiani, D. Caruso. - In: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. - ISSN 0021-8561. - 58:4(2010), pp. 2246-2252. [10.1021/jf9042503]

Olive Oil phenols modulate the expression of metalloproteinase 9 in THP-1 cells by acting on nuclear factor-kB signaling

M. Dell'Agli
Primo
;
R. Fagnani
Secondo
;
G. Galli;O. Maschi;F. Gilardi;S. Bellosta;M. Crestani;E. Bosisio;E. De Fabiani
Penultimo
;
D. Caruso
Ultimo
2010

Abstract

In vivo studies suggest that the phenolic component contributes to the anti-inflammatory and antiatherosclerotic actions of olive oil; however, the effects In circulating cells are not fully characterized. Monocytes play a key role in inflammation-based diseases by expressing several molecules, Including metalloproteinases (MMPs). In the present study, we investigated the effects of olive oil phenolic extract and Individual compounds on MMP-9 in THP-1 cells, a human monocyte-like cell line. Olive oil extract prevented the stimulation of MMP-9 expression and secretion in tumor necrosis factor α-treated THP-1 cells. Oleuropein aglycone, a typical olive oil phenol, was active at concentrations found in the extract, although other compounds probably contribute to the biological activity. We also found that the effect of the extract and individual compounds on MMP-9 is due to impaired nuclear factor-kB signaling. Our findings provide further evidence on the mechanisms by which olive oil reduces the inflammatory burden associated with disorders, such as atherosclerosis.
Settore BIO/14 - Farmacologia
Settore BIO/10 - Biochimica
Settore BIO/15 - Biologia Farmaceutica
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/139730
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