Different epidemiological studies on eastern population have suggested an inverse correlation between consumption of soybean products, source of isoflavones such as daidzein (D) and genistein (G), and development of some chronic diseases. It has been supposed that the mechanism of D and G could be related to their estrogenic or antioxidant activities although the latter has not been extensively investigated. The main objective of the present study was to evaluate the antioxidant activity of D and G in ex-vivo, using human primary lymphocytes separated from peripheric blood of healthy subjects. To this aim we supplemented cells with D and G at different concentrations between 0,01 μM and 2,5 μM, for 24 hours; we chose this range considering that plasma concentration of isoflavones, in not habitual consumers of soybeans products is 0,04 μM, while in habitual consumers it is 6 μM. After supplementation we induced oxidative stress to DNA by treatment with H2O2 500 μM, and to cellular membrane by treatment with Fe2+ 100 μM. DNA and lipid oxidative damages were evaluated by the Comet Assay and MDA quantification in HPLC, respectively. The results obtained in our conditions demonstrated that the concentrations between 2,5 μM and 0,05 μM of D and between 2,5 μM and 0,1 μM of G significantly decreased oxidative damage to DNA with respect to control cells (P<0.05): the concentration of 2,5 μM D and G seemed to offer most protection. Differently, the preventive effect of D and G against oxidative damage to cellular membrane has been demonstrated only with supplementation of 2,5 μM (P<0.05). In conclusion these findings are consistent with an antioxidant activity of D and G in human primary lymphocytes especially with respect to DNA oxidative damage. Furthermore, since the protective effect of these isoflavones has been found with concentrations reachable in plasma of not habitual consumers of soybeans, it seems possible suggest a functional role of these compounds also in western population.

Antioxidant Effect of Daidzein and Genistein in Human Primary Lymphocytes / D. Erba, P. Foti, A. Spadafranca, G. Testolin. - In: ANNALS OF NUTRITION AND METABOLISM. - ISSN 0250-6807. - 47:6(2003), pp. 402-402. ((Intervento presentato al 9. convegno European Nutrition Conference tenutosi a Roma Italy nel 2003.

Antioxidant Effect of Daidzein and Genistein in Human Primary Lymphocytes

D. Erba;P. Foti;A. Spadafranca;G. Testolin
2003

Abstract

Different epidemiological studies on eastern population have suggested an inverse correlation between consumption of soybean products, source of isoflavones such as daidzein (D) and genistein (G), and development of some chronic diseases. It has been supposed that the mechanism of D and G could be related to their estrogenic or antioxidant activities although the latter has not been extensively investigated. The main objective of the present study was to evaluate the antioxidant activity of D and G in ex-vivo, using human primary lymphocytes separated from peripheric blood of healthy subjects. To this aim we supplemented cells with D and G at different concentrations between 0,01 μM and 2,5 μM, for 24 hours; we chose this range considering that plasma concentration of isoflavones, in not habitual consumers of soybeans products is 0,04 μM, while in habitual consumers it is 6 μM. After supplementation we induced oxidative stress to DNA by treatment with H2O2 500 μM, and to cellular membrane by treatment with Fe2+ 100 μM. DNA and lipid oxidative damages were evaluated by the Comet Assay and MDA quantification in HPLC, respectively. The results obtained in our conditions demonstrated that the concentrations between 2,5 μM and 0,05 μM of D and between 2,5 μM and 0,1 μM of G significantly decreased oxidative damage to DNA with respect to control cells (P<0.05): the concentration of 2,5 μM D and G seemed to offer most protection. Differently, the preventive effect of D and G against oxidative damage to cellular membrane has been demonstrated only with supplementation of 2,5 μM (P<0.05). In conclusion these findings are consistent with an antioxidant activity of D and G in human primary lymphocytes especially with respect to DNA oxidative damage. Furthermore, since the protective effect of these isoflavones has been found with concentrations reachable in plasma of not habitual consumers of soybeans, it seems possible suggest a functional role of these compounds also in western population.
Settore BIO/09 - Fisiologia
Federation of European Nutrition Societies
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/12977
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