The release of antimicrobial peptides and growth factors by Paneth cells is thought to play an important role in protecting the small intestine, but the mechanisms involved have remained obscure. Immunohistochemistry and immunofluorescence showed that Paneth cells express Toll-like receptor 9 (TLR9) in the granules. Injection of mice with oligonucleotides containing CpG sequence (CpG-ODNs) led to a down-modulation of TLR9 and a striking decrease in the number of large secretory granules, consistent with degranulation. Moreover CpG-ODN treatment increased resistance to oral challenge with virulent Salmonella typhimurium. Moreover, our findings demonstrate a sentinel role for Paneth cells through TLR9.

Degranulation of paneth cells via toll-like receptor 9 / C. Rumio, D. Besusso, M. Palazzo, S. Selleri, L. Sfondrini, F. Dubini, S. Menard, A. Balsari. - In: THE AMERICAN JOURNAL OF PATHOLOGY. - ISSN 0002-9440. - 165:2(2004), pp. 373-381. [10.1016/S0002-9440(10)63304-4]

Degranulation of paneth cells via toll-like receptor 9

C. Rumio;D. Besusso;M. Palazzo;S. Selleri;L. Sfondrini;F. Dubini;A. Balsari
2004

Abstract

The release of antimicrobial peptides and growth factors by Paneth cells is thought to play an important role in protecting the small intestine, but the mechanisms involved have remained obscure. Immunohistochemistry and immunofluorescence showed that Paneth cells express Toll-like receptor 9 (TLR9) in the granules. Injection of mice with oligonucleotides containing CpG sequence (CpG-ODNs) led to a down-modulation of TLR9 and a striking decrease in the number of large secretory granules, consistent with degranulation. Moreover CpG-ODN treatment increased resistance to oral challenge with virulent Salmonella typhimurium. Moreover, our findings demonstrate a sentinel role for Paneth cells through TLR9.
Settore MED/04 - Patologia Generale
Settore MED/07 - Microbiologia e Microbiologia Clinica
Settore BIO/16 - Anatomia Umana
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/12452
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