The p73 gene has a complex regulation, which leads to the expression of different isoforms, often with opposite biological effects. We have generated in the human colocarcinoma cell line HCT116, expressing a wild-type p53, an inducible DNp73(alpha) expressing system. Two clones (HCT116/DN3 and HCT116/DN14), upon doxycycline addition, show a strong expression of DNp73(alpha). In vitro the two DNp73(alpha) overexpressing clones grow at similar rate of the control transfected clone (HCT116/8a) and similarly respond to DNA damage. When injected in mice, HCT116/DN3, HCT116/DN14, and HCT116/8a cells grew similarly in the absence or presence of tetracycline. In HCT116/DN3 and HCT116/DN14 tumors, tetracycline induced a strong expression of DNp73(alpha) both as mRNA and protein. These results indicate that in this system the overexpression of the DNp73(alpha) does not induce a more aggressive phenotype and does not seem to be associated with a reduced response of the cells to treatment with anticancer agents. (copyright) 2005 Nature Publishing Group. All rights reserved.

Effects of inducible overexpression of DNp73(alpha) on cancer cell growth and response to treatment in vitro and in vivo / F. Polato, M. Marabese, E. Scanziani, E. Riccardi, F. Vikhanskaya, M. Broggini, M.A. Sabatino, S. Marchini, E. Marrazzo. - In: CELL DEATH AND DIFFERENTIATION. - ISSN 1350-9047. - 12:7(2005), pp. 805-814.

Effects of inducible overexpression of DNp73(alpha) on cancer cell growth and response to treatment in vitro and in vivo

E. Scanziani;E. Riccardi;
2005

Abstract

The p73 gene has a complex regulation, which leads to the expression of different isoforms, often with opposite biological effects. We have generated in the human colocarcinoma cell line HCT116, expressing a wild-type p53, an inducible DNp73(alpha) expressing system. Two clones (HCT116/DN3 and HCT116/DN14), upon doxycycline addition, show a strong expression of DNp73(alpha). In vitro the two DNp73(alpha) overexpressing clones grow at similar rate of the control transfected clone (HCT116/8a) and similarly respond to DNA damage. When injected in mice, HCT116/DN3, HCT116/DN14, and HCT116/8a cells grew similarly in the absence or presence of tetracycline. In HCT116/DN3 and HCT116/DN14 tumors, tetracycline induced a strong expression of DNp73(alpha) both as mRNA and protein. These results indicate that in this system the overexpression of the DNp73(alpha) does not induce a more aggressive phenotype and does not seem to be associated with a reduced response of the cells to treatment with anticancer agents. (copyright) 2005 Nature Publishing Group. All rights reserved.
Anticancer agents; In vivo; p53; p73
Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria
CELL DEATH AND DIFFERENTIATION
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/11393
Citazioni
  • ???jsp.display-item.citation.pmc??? 9
  • Scopus 18
  • ???jsp.display-item.citation.isi??? 18
social impact