Implementation of onsite bioremediation technologies is essential for textile industries due to rising concerns in terms of water resources and quality. Partial nitritation-anaerobic ammonium oxidation (PN/A) processes emerged as a valid, but unexplored, solution. In this study, the performance of a PN/A pilot-scale (9 m3) sequencing batch reactor treating digital textile printing wastewater (10–40 m3 d−1) was monitored by computing nitrogen (N) removal rate and efficiencies. Moreover, the structure of the bacterial community was assessed by next generation sequencing and quantitative polymerase chain reaction (qPCR) analyses of several genes, which are involved in the N cycle. Although anaerobic ammonium oxidation activity was inhibited and denitrification occurred, N removal rate increased from 16 to 61 mg N g VSS−1 d−1 reaching satisfactory removal efficiency (up to 70%). Ammonium (18–70 mg L−1) and nitrite (16–82 mg L−1) were detected in the effluent demonstrating an unbalance between the aerobic and anaerobic ammonia oxidation activity, while constant organic N was attributed to recalcitrant azo dyes. Ratio between nitrification and anammox genes remained stable reflecting a constant ammonia oxidation activity. A prevalence of ammonium oxidizing bacteria and denitrifiers suggested the presence of alternative pathways. PN/A resulted a promising cost-effective alternative for textile wastewater N treatment as shown by the technical-economic assessment. However, operational conditions and design need further tailoring to promote the activity of the anammox bacteria.

Microbial community and performance of a partial nitritation/anammox sequencing batch reactor treating textile wastewater / E. Clagnan, L. Brusetti, S. Pioli, S. Visigalli, A. Turolla, M. Jia, M. Bargna, E. Ficara, G. Bergna, R. Canziani, M. Bellucci. - In: HELIYON. - ISSN 2405-8440. - 7:11(2021), pp. e08445.1-e08445.10. [10.1016/j.heliyon.2021.e08445]

Microbial community and performance of a partial nitritation/anammox sequencing batch reactor treating textile wastewater

E. Clagnan
Primo
;
L. Brusetti;S. Visigalli;M. Bellucci
Ultimo
2021

Abstract

Implementation of onsite bioremediation technologies is essential for textile industries due to rising concerns in terms of water resources and quality. Partial nitritation-anaerobic ammonium oxidation (PN/A) processes emerged as a valid, but unexplored, solution. In this study, the performance of a PN/A pilot-scale (9 m3) sequencing batch reactor treating digital textile printing wastewater (10–40 m3 d−1) was monitored by computing nitrogen (N) removal rate and efficiencies. Moreover, the structure of the bacterial community was assessed by next generation sequencing and quantitative polymerase chain reaction (qPCR) analyses of several genes, which are involved in the N cycle. Although anaerobic ammonium oxidation activity was inhibited and denitrification occurred, N removal rate increased from 16 to 61 mg N g VSS−1 d−1 reaching satisfactory removal efficiency (up to 70%). Ammonium (18–70 mg L−1) and nitrite (16–82 mg L−1) were detected in the effluent demonstrating an unbalance between the aerobic and anaerobic ammonia oxidation activity, while constant organic N was attributed to recalcitrant azo dyes. Ratio between nitrification and anammox genes remained stable reflecting a constant ammonia oxidation activity. A prevalence of ammonium oxidizing bacteria and denitrifiers suggested the presence of alternative pathways. PN/A resulted a promising cost-effective alternative for textile wastewater N treatment as shown by the technical-economic assessment. However, operational conditions and design need further tailoring to promote the activity of the anammox bacteria.
Next-generation sequencing; Nitrogen removal; Partial nitritation/anammox; Quantitative polymerase chain reaction (qPCR); Textile wastewater
Settore AGR/13 - Chimica Agraria
Settore AGR/16 - Microbiologia Agraria
Settore AGRI-06/B - Chimica agraria
Settore AGRI-08/A - Microbiologia agraria, alimentare e ambientale
2021
Article (author)
File in questo prodotto:
File Dimensione Formato  
1-s2.0-S2405844021025482-main.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 1.57 MB
Formato Adobe PDF
1.57 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/1116067
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 14
  • ???jsp.display-item.citation.isi??? 13
social impact