Forensic toxicology backdates the use of coca plant (Erythroxylum spp.) in Europe to the early 1600s

Cocaine hydrochloride salts are one of the most commonly used drugs of our days, yet there is very little hard evidence regarding when people started consuming such an extensively popular drug in Europe. In this paper, we report the exceptional finding of Erythroxylum spp. in human remains dated to the 1600's in Milan, Italy. Toxicological analyses were performed on preserved human brains revealing the first evidence of Erythroxylum spp. use in Europe before the 19th century, backdating our understanding of the presence of the plant by almost two centuries. Specifically, the alkaloid of cocaine was detected in two separate biological samples and can be associated to Erythroxylum spp. consumption. Given that the plant was not listed inside the detailed hospital pharmacopeia, it may not have been given as a medicinal remedy but may have been used for other purposes. This study demonstrates the importance and the potential of the application of toxicological analyses to archaeological contexts and allows to backdate the arrival of the Erythroxylum spp. in Europe by almost two hundred years.