Due to its endocrine disruptive activity, the plastic additive Bisphenol A (BPA) is classified as substance of very high concern (EU ECHA 2017). A correlation between environmental exposure to BPA and congenital defects has been described in humans and in experimental species including the amphibian Xenopus laevis, where severe branchial defects were associated to lethality. The exposure of X. laevis embryos to the BPA analogue bisphenol B (BPB) was recently linked to similar teratogenic effects, with BPB having relative potency about 3 times higher than BPA. The combined BPA-BPB exposure is realistic as both BPA and BPB are detected in human samples and environment. Limited experimental data are available on the combined developmental toxicity of BPA and BPB. The aim of the present work is to evaluate the effects of BPA and BPB mixture in the X. laevis development model, using R-FETAX procedure. The exposure was limited to the first day of development (corresponding to the phylotypic developmental period, common to all vertebrates). Samples were monitored for lethal effects during the full six-day test period and the external morphology was evaluated at the end of the test. Mixture effects were described by modelling, using the PROAST software package. Overall data modelling showed that dose-addiction could not be rejected, suggesting a health concern for co-exposure.

Effects of combined exposure to two bisphenol plasticizers (BPA and BPB) on Xenopus laevis development / M. Battistoni, F. Metruccio, F. Di Renzo, A. Moretto, R. Bacchetta, E. Menegola. - In: REPRODUCTIVE TOXICOLOGY. - ISSN 0890-6238. - 128:(2024), pp. 108614.1-108614.8. [10.1016/j.reprotox.2024.108614]

Effects of combined exposure to two bisphenol plasticizers (BPA and BPB) on Xenopus laevis development

M. Battistoni
Primo
;
F. Metruccio;F. Di Renzo
;
A. Moretto;R. Bacchetta
Penultimo
;
E. Menegola
Ultimo
2024

Abstract

Due to its endocrine disruptive activity, the plastic additive Bisphenol A (BPA) is classified as substance of very high concern (EU ECHA 2017). A correlation between environmental exposure to BPA and congenital defects has been described in humans and in experimental species including the amphibian Xenopus laevis, where severe branchial defects were associated to lethality. The exposure of X. laevis embryos to the BPA analogue bisphenol B (BPB) was recently linked to similar teratogenic effects, with BPB having relative potency about 3 times higher than BPA. The combined BPA-BPB exposure is realistic as both BPA and BPB are detected in human samples and environment. Limited experimental data are available on the combined developmental toxicity of BPA and BPB. The aim of the present work is to evaluate the effects of BPA and BPB mixture in the X. laevis development model, using R-FETAX procedure. The exposure was limited to the first day of development (corresponding to the phylotypic developmental period, common to all vertebrates). Samples were monitored for lethal effects during the full six-day test period and the external morphology was evaluated at the end of the test. Mixture effects were described by modelling, using the PROAST software package. Overall data modelling showed that dose-addiction could not be rejected, suggesting a health concern for co-exposure.
Data modelling; Development; Dose-addition; Embryotoxicity; Endocrine disruptors; Mixture
Settore BIO/06 - Anatomia Comparata e Citologia
   Assessing the health risks of combined human exposure to multiple food-related toxic substances
   EuroMix
   EUROPEAN COMMISSION
   H2020
   633172
2024
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/1064488
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