Serratia marcescens is an opportunistic pathogen that survives in inhospitable environments causing large outbreaks, particularly in neonatal intensive care units (NICUs). Genomic studies revealed that most S. marcescens nosocomial infections are caused by a specific clone (here "Infectious clone"). Whole genome sequencing (WGS) is the only portable method able to identify this clone, but it requires days to obtain results. We present a cultivation-free hypervariable-locus melting typing (HLMT) protocol for the fast detection and typing of S. marcescens, with 100% detection capability on mixed samples and a limit of detection that can reach the 10 genome copies. The protocol was able to identify the S. marcescens infectious clone with 97% specificity and 96% sensitivity when compared to WGS, yielding typing results portable among laboratories. The protocol is a cost and time saving method for S. marcescens detection and typing for large environmental/clinical surveillance screenings, also in low-middle income countries.

Cultivation and sequencing-free protocol for Serratia marcescens detection and typing / A. Alvaro, A. Piazza, S. Papaleo, M. Perini, A.R. Pasala, S. Panelli, T. Nardi, R. Nodari, L. Sterzi, C. Pagani, C. Merla, D. Castelli, E. Olivieri, S. Bracco, M. Laura Ferrando, F. Saluzzo, S.G. Rimoldi, M. Corbella, A. Cavallero, P. Prati, C. Farina, D. Maria Cirillo, G. Zuccotti, F. Comandatore. - In: ISCIENCE. - ISSN 2589-0042. - 27:4(2024 Apr 19), pp. 109402.1-109402.13. [10.1016/j.isci.2024.109402]

Cultivation and sequencing-free protocol for Serratia marcescens detection and typing

A. Alvaro
Primo
;
A. Piazza
Secondo
;
S. Papaleo;M. Perini;A.R. Pasala;S. Panelli;T. Nardi;R. Nodari;L. Sterzi;C. Pagani;D. Castelli;S.G. Rimoldi;G. Zuccotti
Penultimo
;
F. Comandatore
Ultimo
2024

Abstract

Serratia marcescens is an opportunistic pathogen that survives in inhospitable environments causing large outbreaks, particularly in neonatal intensive care units (NICUs). Genomic studies revealed that most S. marcescens nosocomial infections are caused by a specific clone (here "Infectious clone"). Whole genome sequencing (WGS) is the only portable method able to identify this clone, but it requires days to obtain results. We present a cultivation-free hypervariable-locus melting typing (HLMT) protocol for the fast detection and typing of S. marcescens, with 100% detection capability on mixed samples and a limit of detection that can reach the 10 genome copies. The protocol was able to identify the S. marcescens infectious clone with 97% specificity and 96% sensitivity when compared to WGS, yielding typing results portable among laboratories. The protocol is a cost and time saving method for S. marcescens detection and typing for large environmental/clinical surveillance screenings, also in low-middle income countries.
Biological sciences; Biological sciences research methodologies; Environmental Biotechnology; Microbiology
Settore MED/07 - Microbiologia e Microbiologia Clinica
Settore BIO/19 - Microbiologia Generale
19-apr-2024
25-lug-2023
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/1055988
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