Biological ion channels precisely control the flow of ions across membranes in response to a range of physical and chemical stimuli. With their ability of transporting ions in a highly selective manner and of integrating regulatory cues, they are a source of inspiration for the construction of solid-state nanopores as sensors or switches for practical applications. Here, we summarize recent advancements in understanding the mechanisms of ion permeation and gating in channel proteins with a focus on the elementary steps of ion transport through the pore and on non-canonical modes of intramolecular communication between peripheral sensory domains and the central channel pore.

Experimental challenges in ion channel research: uncovering basic principles of permeation and gating in potassium channels / J.L. Carvalho-de-Souza, A. Saponaro, C.A.Z. Bassetto, O. Rauh, I. Schroeder, F. Franciolini, L. Catacuzzeno, F. Bezanilla, G. Thiel, A. Moroni. - In: ADVANCES IN PHYSICS: X. - ISSN 2374-6149. - 7:1(2022 Jan), pp. 1978317.1-1978317.24. [10.1080/23746149.2021.1978317]

Experimental challenges in ion channel research: uncovering basic principles of permeation and gating in potassium channels

A. Saponaro
Secondo
;
A. Moroni
Ultimo
2022

Abstract

Biological ion channels precisely control the flow of ions across membranes in response to a range of physical and chemical stimuli. With their ability of transporting ions in a highly selective manner and of integrating regulatory cues, they are a source of inspiration for the construction of solid-state nanopores as sensors or switches for practical applications. Here, we summarize recent advancements in understanding the mechanisms of ion permeation and gating in channel proteins with a focus on the elementary steps of ion transport through the pore and on non-canonical modes of intramolecular communication between peripheral sensory domains and the central channel pore.
K+ channel; gating; voltage sensor; selectivity filter; intramolecular communication
Settore BIO/09 - Fisiologia
   Noninvasive Manipulation of Gating in Ion Channels
   noMAGIC
   EUROPEAN COMMISSION
   H2020
   695078

   The crucial role of HCN2 channels in inflammatory and neuropathic pain
   FONDAZIONE CARIPLO
   2018-
gen-2022
15-ott-2021
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/1049599
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