Both viral infection and vaccination affect the antibody repertoire of a person. Here, we demonstrate that the analysis of serum antibodies generates information not only on the virus type that caused the infection but also on the specific virus variant. We developed a rapid multiplex assay providing a fingerprint of serum antibodies against five different SARS-CoV-2 variants based on a microarray of virus antigens immobilized on the surface of a label- free reflectometric biosensor. We analyzed serum from the plasma of convalescent subjects and vaccinated volunteers and extracted individual antibody profiles of both total immunoglobulin Ig and IgA fractions. We found that Ig level profiles were strongly correlated with the specific variant of infection or vaccination and that vaccinated subjects displayed a larger quantity of total Ig and a lower fraction of IgA relative to the population of convalescent unvaccinated subjects.

Serum antibody fingerprinting of SARS-CoV-2 variants in infected and vaccinated subjects by label-free microarray biosensor / T. Carzaniga, L. Casiraghi, G. Nava, G. Zanchetta, T. Inzani, M. Chiari, V. Bollati, S. Epis, C. Bandi, A. Lai, G. Zehender, T. Bellini, M. Buscaglia. - In: FRONTIERS IN IMMUNOLOGY. - ISSN 1664-3224. - 15:(2024 Feb), pp. 1323406.1-1323406.11. [10.3389/fimmu.2024.1323406]

Serum antibody fingerprinting of SARS-CoV-2 variants in infected and vaccinated subjects by label-free microarray biosensor

T. Carzaniga
Co-primo
;
L. Casiraghi
Co-primo
;
G. Nava;G. Zanchetta;V. Bollati;S. Epis;C. Bandi;A. Lai;G. Zehender;T. Bellini
Penultimo
;
M. Buscaglia
Ultimo
2024

Abstract

Both viral infection and vaccination affect the antibody repertoire of a person. Here, we demonstrate that the analysis of serum antibodies generates information not only on the virus type that caused the infection but also on the specific virus variant. We developed a rapid multiplex assay providing a fingerprint of serum antibodies against five different SARS-CoV-2 variants based on a microarray of virus antigens immobilized on the surface of a label- free reflectometric biosensor. We analyzed serum from the plasma of convalescent subjects and vaccinated volunteers and extracted individual antibody profiles of both total immunoglobulin Ig and IgA fractions. We found that Ig level profiles were strongly correlated with the specific variant of infection or vaccination and that vaccinated subjects displayed a larger quantity of total Ig and a lower fraction of IgA relative to the population of convalescent unvaccinated subjects.
label-free biosensor, reflective phantom interface, antibody repertoire, rapid detection, wash-free assay, serological assay, immunoglobulins, IgA
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
   SOFT ADAPTIVE NETWORKS
   MINISTERO DELL'ISTRUZIONE E DEL MERITO
   2017Z55KCW_002

   Test rapido di contagiosità da COVID-19: sviluppo di un biosensore per misurare la presenza del virus intero nell'esalato respiratorio (VIAEREA)
   VIAEREA
   MINISTERO DELL'ISTRUZIONE E DEL MERITO
feb-2024
feb-2024
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/1033348
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