The molecular mechanism by which lipid/lipoprotein biosynthesis is regulated in mammals involves a very large number of genes that are subject to multiple levels of regulation. miRNAs are recognized contributors to lipid homeostasis at the post-transcriptional level, although the elucidation of their role is made difficult by the multiplicity of their targets and the ability of more miRNAs to affect the same mRNAs. In this study, an evaluation of how miRNA expression varies in organs playing a key role in lipid/lipoprotein metabolism was conducted in control mice and in two mouse models carrying genetic ablations which differently affect low-density lipoprotein metabolism. Mice were fed a lipid-poor standard diet and a diet enriched in cholesterol and saturated fat. The results obtained showed that there are no miRNAs whose expression constantly vary with dietary or genetic changes. Furthermore, it appears that diet, more than genotype, impacts on organ-specific miRNA expression profiles.

Effect of diet and genotype on the miRNome of mice with altered lipoprotein metabolism / M. Busnelli, S. Manzini, A. Colombo, E. Franchi, M. Chiara, G. Zaffaroni, D. Horner, G. Chiesa. - In: ISCIENCE. - ISSN 2589-0042. - 26:9(2023 Aug 11), pp. 107615.1-107615.19. [10.1016/j.isci.2023.107615]

Effect of diet and genotype on the miRNome of mice with altered lipoprotein metabolism

M. Busnelli
Primo
;
S. Manzini
Secondo
;
A. Colombo;E. Franchi;M. Chiara;D. Horner
Penultimo
;
G. Chiesa
Ultimo
2023

Abstract

The molecular mechanism by which lipid/lipoprotein biosynthesis is regulated in mammals involves a very large number of genes that are subject to multiple levels of regulation. miRNAs are recognized contributors to lipid homeostasis at the post-transcriptional level, although the elucidation of their role is made difficult by the multiplicity of their targets and the ability of more miRNAs to affect the same mRNAs. In this study, an evaluation of how miRNA expression varies in organs playing a key role in lipid/lipoprotein metabolism was conducted in control mice and in two mouse models carrying genetic ablations which differently affect low-density lipoprotein metabolism. Mice were fed a lipid-poor standard diet and a diet enriched in cholesterol and saturated fat. The results obtained showed that there are no miRNAs whose expression constantly vary with dietary or genetic changes. Furthermore, it appears that diet, more than genotype, impacts on organ-specific miRNA expression profiles.
Cellular physiology; Molecular biology; Molecular physiology; Omics
Settore BIO/14 - Farmacologia
Settore BIO/16 - Anatomia Umana
   ERA-Net on INtesTInal MICrobiomics, diet and health, implementing JPI HDHL objectives
   HDHL-INTIMIC
   European Commission
   Horizon 2020 Framework Programme
   727565

   Characterization of microRNA functional role in cholesterol metabolism and in the pathogenesis of atherosclerosis
   FONDAZIONE CARIPLO
   2011-0645

   Assegnazione Dipartimenti di Eccellenza 2023-2027 - Dipartimento di SCIENZE FARMACOLOGICHE E BIOMOLECOLARI
   DECC23_022
   MINISTERO DELL'UNIVERSITA' E DELLA RICERCA
11-ago-2023
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/1020608
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