Background We aimed at evaluating the temporal trend of drug-resistance and APOBEC editing from HIV-DNA genotypic resistance tests (GRT) in virologically suppressed individuals. Material and methods Major resistance mutations (MRM), genotypic susceptibility score (GSS) for the current regimen and APOBEC-related mutations (APO-M) were evaluated. Potential changes in trends of MRM and APO-M over-time were assessed and predictors of MRM detection or sub-optimal GSS (GSS<2) at HIV-DNA-GRT were estimated through logistic regression analyses. Results Among the 1126 individuals included, 396 (35.2%) harboured at least one MRM (23.4% to NRTI, 18.8% to NNRTI, 7.7% to PI and 1.4% to INSTI [N=724]); 132 (12.3%) individuals showed a GSS <2. APO-M and stop codons were found in 229 (20.3%) and 105 (9.3%) individuals, respectively. APO-DRMs were found in 16.8% of individuals and were more likely observed in those individuals with stop codons (40.0%) compared to those without (14.4%, P<0.001). From 2010 to 2021 no significant changes of resistance or APO-M were found. Positive predictors of MRM detection at HIV-DNA GRT were drug abuse, subtype B infection, and a prolonged and complex treatment history. Perinatal infection and having at least 2 stop codons were associated with a current suboptimal regimen. Conclusions In virologically suppressed individuals, resistance in HIV-DNA and the extent of APOBEC editing were generally stable in the last decade. A careful evaluation of APOBEC editing might be helpful to improve the reliability of HIV-DNA GRT. Further investigations are required to understand how to apply the estimation of APOBEC editing in refining genotypic evaluation.

Temporal trend of drug-resistance and APOBEC editing in PBMC genotypic resistance tests from HIV-1 infected virologically suppressed individuals / D. Armenia, R. Gagliardini, C. Alteri, V. Svicher, V. Cento, V. Borghi, A. Vergori, S. Cicalini, F. Forbici, L. Fabeni, A. Bertoli, M. Brugneti, W. Gennari, V. Malagnino, M. Andreoni, C. Mussini, A. Antinori, C.F. Perno, M.M. Santoro, F. Ceccherini-Silberstein. - In: JOURNAL OF CLINICAL VIROLOGY. - ISSN 1386-6532. - (2023), pp. 105551.1-105551.7. [Epub ahead of print] [10.1016/j.jcv.2023.105551]

Temporal trend of drug-resistance and APOBEC editing in PBMC genotypic resistance tests from HIV-1 infected virologically suppressed individuals

C. Alteri;
2023

Abstract

Background We aimed at evaluating the temporal trend of drug-resistance and APOBEC editing from HIV-DNA genotypic resistance tests (GRT) in virologically suppressed individuals. Material and methods Major resistance mutations (MRM), genotypic susceptibility score (GSS) for the current regimen and APOBEC-related mutations (APO-M) were evaluated. Potential changes in trends of MRM and APO-M over-time were assessed and predictors of MRM detection or sub-optimal GSS (GSS<2) at HIV-DNA-GRT were estimated through logistic regression analyses. Results Among the 1126 individuals included, 396 (35.2%) harboured at least one MRM (23.4% to NRTI, 18.8% to NNRTI, 7.7% to PI and 1.4% to INSTI [N=724]); 132 (12.3%) individuals showed a GSS <2. APO-M and stop codons were found in 229 (20.3%) and 105 (9.3%) individuals, respectively. APO-DRMs were found in 16.8% of individuals and were more likely observed in those individuals with stop codons (40.0%) compared to those without (14.4%, P<0.001). From 2010 to 2021 no significant changes of resistance or APO-M were found. Positive predictors of MRM detection at HIV-DNA GRT were drug abuse, subtype B infection, and a prolonged and complex treatment history. Perinatal infection and having at least 2 stop codons were associated with a current suboptimal regimen. Conclusions In virologically suppressed individuals, resistance in HIV-DNA and the extent of APOBEC editing were generally stable in the last decade. A careful evaluation of APOBEC editing might be helpful to improve the reliability of HIV-DNA GRT. Further investigations are required to understand how to apply the estimation of APOBEC editing in refining genotypic evaluation.
No
English
APOBEC editing; Drug-resistance; Genotypic susceptibility score; HIV-DNA GRT; PBMCs; Virological suppression;
Settore MED/07 - Microbiologia e Microbiologia Clinica
Articolo
Esperti anonimi
Pubblicazione scientifica
2023
23-lug-2023
Elsevier
105551
1
7
7
Epub ahead of print
Periodico con rilevanza internazionale
scopus
crossref
Aderisco
info:eu-repo/semantics/article
Temporal trend of drug-resistance and APOBEC editing in PBMC genotypic resistance tests from HIV-1 infected virologically suppressed individuals / D. Armenia, R. Gagliardini, C. Alteri, V. Svicher, V. Cento, V. Borghi, A. Vergori, S. Cicalini, F. Forbici, L. Fabeni, A. Bertoli, M. Brugneti, W. Gennari, V. Malagnino, M. Andreoni, C. Mussini, A. Antinori, C.F. Perno, M.M. Santoro, F. Ceccherini-Silberstein. - In: JOURNAL OF CLINICAL VIROLOGY. - ISSN 1386-6532. - (2023), pp. 105551.1-105551.7. [Epub ahead of print] [10.1016/j.jcv.2023.105551]
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D. Armenia, R. Gagliardini, C. Alteri, V. Svicher, V. Cento, V. Borghi, A. Vergori, S. Cicalini, F. Forbici, L. Fabeni, A. Bertoli, M. Brugneti, W. Ge...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/999810
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