The potential antiviral effects of indole-3-carbinol (I3C), a phytochemical found in Cruciferous vegetables, were investigated. Fibroblasts and epithelial cells were co-cultured on Alvetex (R) scaffolds, to obtain ad hoc 3D in vitro platforms able to mimic the trachea and intestinal mucosae, which represent the primary structures involved in the coronavirus pathogenesis. The two barriers generated in vitro were treated with various concentrations of I3C for different incubation periods. A protective effect of I3C on both intestinal and trachea models was demon-strated. A significant reduction in the transcription of the two main genes belonging to the Homologous to E6AP C-terminus (HECT)-E3 ligase family members, namely NEDD4 E3 Ubiquitin Protein Ligase (NEDD4) and WW Domain Containing E3 Ubiquitin Protein Ligase 1 (WWP1), which promote virus matrix protein ubiquitination and inhibit viral egression, were detected. These findings indicate I3C potential effect in preventing coronavirus cell egression processes that inhibit viral production. Although further studies are needed to clarify the molecular mechanisms whereby HECT family members control virus life cycle, this work paves the way to the possible therapeutic use of new natural com-pounds that may reduce the clinical severity of future pandemics.

Cruciferous vegetable-derived indole-3-carbinol prevents coronavirus cell egression mechanisms in tracheal and intestinal 3D in vitro models / G. Pennarossa, S. Arcuri, R. Pasquariello, F. Gandolfi, M. Maranesi, T. Brevini. - In: PHYTOCHEMISTRY. - ISSN 0031-9422. - 212:(2023 Aug), pp. 113713.1-113713.7. [10.1016/j.phytochem.2023.113713]

Cruciferous vegetable-derived indole-3-carbinol prevents coronavirus cell egression mechanisms in tracheal and intestinal 3D in vitro models

G. Pennarossa
Primo
;
S. Arcuri
Secondo
;
R. Pasquariello;F. Gandolfi;T. Brevini
Ultimo
2023

Abstract

The potential antiviral effects of indole-3-carbinol (I3C), a phytochemical found in Cruciferous vegetables, were investigated. Fibroblasts and epithelial cells were co-cultured on Alvetex (R) scaffolds, to obtain ad hoc 3D in vitro platforms able to mimic the trachea and intestinal mucosae, which represent the primary structures involved in the coronavirus pathogenesis. The two barriers generated in vitro were treated with various concentrations of I3C for different incubation periods. A protective effect of I3C on both intestinal and trachea models was demon-strated. A significant reduction in the transcription of the two main genes belonging to the Homologous to E6AP C-terminus (HECT)-E3 ligase family members, namely NEDD4 E3 Ubiquitin Protein Ligase (NEDD4) and WW Domain Containing E3 Ubiquitin Protein Ligase 1 (WWP1), which promote virus matrix protein ubiquitination and inhibit viral egression, were detected. These findings indicate I3C potential effect in preventing coronavirus cell egression processes that inhibit viral production. Although further studies are needed to clarify the molecular mechanisms whereby HECT family members control virus life cycle, this work paves the way to the possible therapeutic use of new natural com-pounds that may reduce the clinical severity of future pandemics.
3D in vitro models; Coronavirus; Cruciferous; Indole-3-carbinol; Intestine; Trachea
Settore VET/01 - Anatomia degli Animali Domestici
   Boosting the One Health Research Excellence and Management Capacity of the Estonian University of Life Sciences (OH-Boost)
   OH-Boost
   EUROPEAN COMMISSION
   101079349
ago-2023
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/996490
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