A structured chemical platform based on chitosan, an amine-rich polysaccharide, is presented as an alternative chemistry to functionalize solid support (in this case, glass slides) for grafting biomolecules. This approach has been adopted for generating arrays using amino-modified oligonucleotides with two different lengths (25-mer and 70-mer) for different purposes. Results using these chitosan-activated surfaces indicate high oligonucleotide loading capacity, good availability to hybridization against targets, and activeness in enzyme-mediated single nucleotide polymorphism (SNP) detection procedures by DNA polymerase and DNA ligase enzymes with low background. Universal arrays have been prepared and extensively used with excellent results in different applications. The chitosan-treated surfaces were also evaluated for their performance in a gene expression experiment.
A structured chitosan-based platform for biomolecule attachment to solid surfaces: application to DNA microarray preparation / C.Consolandi, M. Severgnini, B.Castiglioni, R. Bordoni, A. Frosini, C. Battaglia, L. Rossi Bernardi, G.D. Bellis. - In: BIOCONJUGATE CHEMISTRY. - ISSN 1043-1802. - 17:2(2006 Apr), pp. 371-377. [10.1021/bc050285a]
A structured chitosan-based platform for biomolecule attachment to solid surfaces: application to DNA microarray preparation
C. Battaglia;L. Rossi Bernardi;
2006
Abstract
A structured chemical platform based on chitosan, an amine-rich polysaccharide, is presented as an alternative chemistry to functionalize solid support (in this case, glass slides) for grafting biomolecules. This approach has been adopted for generating arrays using amino-modified oligonucleotides with two different lengths (25-mer and 70-mer) for different purposes. Results using these chitosan-activated surfaces indicate high oligonucleotide loading capacity, good availability to hybridization against targets, and activeness in enzyme-mediated single nucleotide polymorphism (SNP) detection procedures by DNA polymerase and DNA ligase enzymes with low background. Universal arrays have been prepared and extensively used with excellent results in different applications. The chitosan-treated surfaces were also evaluated for their performance in a gene expression experiment.Pubblicazioni consigliate
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