The determination of the postmortem interval is a topic of great forensic interest. The possibility of using new technologies has allowed the study of postmortem decay of biomolecules in the determination of PMI. Skeletal muscle proteins are promising candidates because skeletal muscle exhibits slower postmortem decay compared to other internal organs and nervous tissues, while its degradation is faster than cartilage and bone. In this pilot study, skeletal muscle tissue from pigs was degraded at two different controlled temperatures, 21 °C and 6 °C, and analysed at predefined times points: 0, 24, 48, 72, 96, and 120 h. The obtained samples were analysed by mass spectrometry proteomics approach for qualitative and quantitative evaluation of proteins and peptides. Immunoblotting validation was performed for the candidate proteins. The results obtained appeared significant and identified several proteins useful for possible postmortem interval estimation. Of these proteins, PDLIM7, TPM1, and ATP2A2 were validated by immunoblotting at a larger number of experimental points and at different temperatures. The results obtained are in agreement with those observed in similar works. In addition, the use of a mass spectrometry approach increased the number of protein species identified, providing a larger panel of proteins for PMI assessment.

Proteomic analysis by mass spectrometry of postmortem muscle protein degradation for PMI estimation: A pilot study / A. Battistini, D. Capitanio, P. Bailo, M. Moriggi, S. Tambuzzi, C. Gelfi, A. Piccinini. - In: FORENSIC SCIENCE INTERNATIONAL. - ISSN 0379-0738. - 349:(2023 Aug), pp. 111774.1-111774.8. [10.1016/j.forsciint.2023.111774]

Proteomic analysis by mass spectrometry of postmortem muscle protein degradation for PMI estimation: A pilot study

A. Battistini
Co-primo
;
D. Capitanio
Co-primo
;
M. Moriggi;S. Tambuzzi;C. Gelfi;A. Piccinini
Ultimo
2023

Abstract

The determination of the postmortem interval is a topic of great forensic interest. The possibility of using new technologies has allowed the study of postmortem decay of biomolecules in the determination of PMI. Skeletal muscle proteins are promising candidates because skeletal muscle exhibits slower postmortem decay compared to other internal organs and nervous tissues, while its degradation is faster than cartilage and bone. In this pilot study, skeletal muscle tissue from pigs was degraded at two different controlled temperatures, 21 °C and 6 °C, and analysed at predefined times points: 0, 24, 48, 72, 96, and 120 h. The obtained samples were analysed by mass spectrometry proteomics approach for qualitative and quantitative evaluation of proteins and peptides. Immunoblotting validation was performed for the candidate proteins. The results obtained appeared significant and identified several proteins useful for possible postmortem interval estimation. Of these proteins, PDLIM7, TPM1, and ATP2A2 were validated by immunoblotting at a larger number of experimental points and at different temperatures. The results obtained are in agreement with those observed in similar works. In addition, the use of a mass spectrometry approach increased the number of protein species identified, providing a larger panel of proteins for PMI assessment.
Degradation; Forensic; Mass spectrometry; Muscle; PMI; Protein
Settore MED/43 - Medicina Legale
ago-2023
27-giu-2023
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/983056
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