Micronucleus (MN) is regarded as an abnormal structure in eukaryotic cells which can be used as a biomarker for genetic instability. However, direct observation of MN in living cells is rarely achieved due to the lack of probes that are capable of distinguishing nuclear- and MN-DNA. Herein, a water-soluble terpyridine organic small molecule (ABT) was designed and employed to recognize Zinc-finger protein (ZF) for imaging intracellular MN. The in vitro experiments suggested ABT has a high affinity towards ZF. Further live cell staining showed that ABT could selectively target MN in HeLa and NSC34 cells when combined with ZF. Importantly, we use ABT to uncover the correlation between neurotoxic amyloid β-protein (Aβ) and MN during Alzheimer's disease (AD) progression. Thus, this study provides profound insight into the relationship between Aβ and genomic disorders, offering a deeper understanding for the diagnosis and treatment of AD.

Revealing the amyloid β-protein with zinc finger protein of micronucleus during Alzheimer's disease progress by a quaternary ammonium terpyridine probe / H. Ding, S. Liu, W. Du, L. Su, J. Chen, Y. Tian, D. Pan, L. Chen, L. Rizzello, X. Zheng, G. Battaglia, K. Luo, Q. Gong, X. Tian. - In: BIOSENSORS & BIOELECTRONICS. - ISSN 1873-4235. - 236:(2023 Sep 15), pp. 115446.1-115446.8. [10.1016/j.bios.2023.115446]

Revealing the amyloid β-protein with zinc finger protein of micronucleus during Alzheimer's disease progress by a quaternary ammonium terpyridine probe

L. Rizzello;
2023

Abstract

Micronucleus (MN) is regarded as an abnormal structure in eukaryotic cells which can be used as a biomarker for genetic instability. However, direct observation of MN in living cells is rarely achieved due to the lack of probes that are capable of distinguishing nuclear- and MN-DNA. Herein, a water-soluble terpyridine organic small molecule (ABT) was designed and employed to recognize Zinc-finger protein (ZF) for imaging intracellular MN. The in vitro experiments suggested ABT has a high affinity towards ZF. Further live cell staining showed that ABT could selectively target MN in HeLa and NSC34 cells when combined with ZF. Importantly, we use ABT to uncover the correlation between neurotoxic amyloid β-protein (Aβ) and MN during Alzheimer's disease (AD) progression. Thus, this study provides profound insight into the relationship between Aβ and genomic disorders, offering a deeper understanding for the diagnosis and treatment of AD.
Alzheimer's disease; Amyloid β-protein; Bioimaging; Micronuclei; Zinc finger protein;
Settore BIO/10 - Biochimica
Settore BIO/11 - Biologia Molecolare
Settore CHIM/09 - Farmaceutico Tecnologico Applicativo
Settore BIO/14 - Farmacologia
15-set-2023
3-giu-2023
Article (author)
File in questo prodotto:
File Dimensione Formato  
1-s2.0-S0956566323003883-main(4).pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 6.65 MB
Formato Adobe PDF
6.65 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
ABT-probe-manuscript.pdf

embargo fino al 15/09/2025

Tipologia: Post-print, accepted manuscript ecc. (versione accettata dall'editore)
Dimensione 1.1 MB
Formato Adobe PDF
1.1 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/976808
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 1
social impact