Hepatitis B virus (HBV) strains isolated worldwide have been classified into 8 genomic groups (A to H) defined by an intergroup divergence of more than 8% in the complete genome sequence. The genotypes show a geographical pattern in their distribution and may influence the natural course of chronic HBV infection. To determine HBV distribution and correlation with particular risk behaviours among individuals living in Italy, the serum samples from 62 subjects with chronic HBV infection and 55 patients with HBV/HIV-1 co-infection were examined for the presence of HBV-DNA by amplifying a highly conserved S gene region through nested-PCR assay and for genotypes by sequence analysis of a 1182 bp fragment including entirely S, pre-S2 and partially pre-S1 regions. Ninety-eight out of 117 (83.8%) patients were HBV-DNA positive and all samples were classified by sequencing. Genotype D was the most prevalent both in chronic HBV infected (59.7%) and in HBV/HIV-1 co-infected (47.2%) patients, followed by genotype A (25.8% and 36.1%, respectively). Genotype F was detected in 7.2%, genotype G in 5.1%, while genotypes B, C and E in 1.0% respectively of the study population. Genotypes A, D, F and G were present in Italian-born subjects, whereas genotypes B, C and E were found in subjects from Asia and Africa. As regard to risk factors, we found a significative association between genotype D and parenteral route, whereas genotype A seems to be related to sexual route of transmission. Further investigations should be carried out on larger number of samples in order to better understand the HBV genotypes distribution in the Italian population.
|Titolo:||Hepatitis B virus genotypes distribution in individuals with chronic HBV infection and in patients with HBV/HIV-1 co-infection living in Italy|
|Autori interni:||TANZI, ELISABETTA (Primo)|
ZAPPA, ALESSANDRA (Secondo)
ZEHENDER, GIANGUGLIELMO (Ultimo)
DE MADDALENA, CHIARA
|Parole Chiave:||Genotypes; HBV; HBV/HIV-1|
|Settore Scientifico Disciplinare:||Settore MED/17 - Malattie Infettive|
Settore MED/42 - Igiene Generale e Applicata
|Data di pubblicazione:||dic-2005|
|Appare nelle tipologie:||01 - Articolo su periodico|
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