Serotonin (5-hydroxytryptamine (5-HT)) is a biogenic monoamine with pleiotropic functions. It exerts its roles by binding to specific 5-HT receptors (5HTRs) classified into different families and subtypes. Homologs of 5HTRs are widely present in invertebrates, but their expression and pharmacological characterization have been scarcely investigated. In particular, 5-HT has been localized in many tunicate species but only a few studies have investigated its physiological functions. Tunicates, including ascidians, are the sister group of vertebrates, and data about the role of 5-HTRs in these organisms are thus important for understanding 5-HT evolution among animals. In the present study, we identified and described 5HTRs in the ascidian Ciona intestinalis. During development, they showed broad expression patterns that appeared consistent with those reported in other species. Then, we investigated 5-HT roles in ascidian embryogenesis exposing C. intestinalis embryos to WAY-100635, an antagonist of the 5HT1A receptor, and explored the affected pathways in neural development and melanogenesis. Our results contribute to unraveling the multifaceted functions of 5-HT, revealing its involvement in sensory cell differentiation in ascidians.

Serotonin Receptors and Their Involvement in Melanization of Sensory Cells in Ciona intestinalis / S. Mercurio, M. Bozzo, A. Pennati, S. Candiani, R. Pennati. - In: CELLS. - ISSN 2073-4409. - 12:8(2023), pp. 1150.1-1150.16. [10.3390/cells12081150]

Serotonin Receptors and Their Involvement in Melanization of Sensory Cells in Ciona intestinalis

S. Mercurio;R. Pennati
Ultimo
2023

Abstract

Serotonin (5-hydroxytryptamine (5-HT)) is a biogenic monoamine with pleiotropic functions. It exerts its roles by binding to specific 5-HT receptors (5HTRs) classified into different families and subtypes. Homologs of 5HTRs are widely present in invertebrates, but their expression and pharmacological characterization have been scarcely investigated. In particular, 5-HT has been localized in many tunicate species but only a few studies have investigated its physiological functions. Tunicates, including ascidians, are the sister group of vertebrates, and data about the role of 5-HTRs in these organisms are thus important for understanding 5-HT evolution among animals. In the present study, we identified and described 5HTRs in the ascidian Ciona intestinalis. During development, they showed broad expression patterns that appeared consistent with those reported in other species. Then, we investigated 5-HT roles in ascidian embryogenesis exposing C. intestinalis embryos to WAY-100635, an antagonist of the 5HT1A receptor, and explored the affected pathways in neural development and melanogenesis. Our results contribute to unraveling the multifaceted functions of 5-HT, revealing its involvement in sensory cell differentiation in ascidians.
G-protein-coupled receptors; ascidian; bipolar tail neurons; epidermal sensory neurons; melanin synthesis; peripheral nervous system; pigment; pigmented sensory organs; serotonin evolution; tunicate
Settore BIO/05 - Zoologia
Settore BIO/06 - Anatomia Comparata e Citologia
   Ageing, Regeneration, and Drug Research
   ARDRE
   European Commission
   Horizon 2020 Framework Programme
   847681
2023
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/972908
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