Purpose: Persistence of skin and wound infections is nowadays accepted being linked to bacterial biofilms, which are highly recalcitrant to treatments and contribute to maintain a constant inflammation state and prevent a correct healing. Topical antimicrobials are the most common first-line self-medications; however, treatment failure is not uncommon and emerging resistance to antibiotics is alarming. Chlorquinaldol is an antimicrobial with a wide spectrum of activity and desirable characteristics for topical application. Aim of this study was to evaluate the efficacy of chlorquinaldol to prevent or eradicate S. aureus and P. aeruginosa biofilms, in comparison to classic topical antibiotics like gentamicin and fusidic acid.Methods: Minimum inhibitory concentrations (MIC) were assessed for each strain and subinhibitory concentrations (1/2 and 1/4 MIC) were used in the biofilm assay. Antimicrobial assays were performed during biofilm formation or were applied on mature biofilms and were evaluated by means of crystal violet assay and confocal laser scan microscopy.Results: Chlorquinaldol and gentamicin were the most effective antimicrobials in both eradicating and preventing pathogens biofilm; however, resistance to methicillin and impermeability to carbapenems impaired chlorquinaldol effect. In addition, similarly to other hydroxyquinolines, aspecific metal chelation is here proposed as chlorquinaldol mode of action.Conclusion: Relying on an acceptable antibiofilm and a wide spectrum of activity, an aspecific mode of action and consequent absence of resistance development, chlorquinaldol proved to be a good antimicrobial for topical use.

Chlorquinaldol, a topical agent for skin and wound infections: anti-biofilm activity and biofilm-related antimicrobial cross-resistance / A. Bidossi, M. Bottagisio, R. De Grandi, L. Drago, E. DE VECCHI. - In: INFECTION AND DRUG RESISTANCE. - ISSN 1178-6973. - 12:(2019), pp. 2177-2189. [10.2147/IDR.S211007]

Chlorquinaldol, a topical agent for skin and wound infections: anti-biofilm activity and biofilm-related antimicrobial cross-resistance

M. Bottagisio
Secondo
;
L. Drago
Penultimo
;
E. DE VECCHI
Ultimo
2019

Abstract

Purpose: Persistence of skin and wound infections is nowadays accepted being linked to bacterial biofilms, which are highly recalcitrant to treatments and contribute to maintain a constant inflammation state and prevent a correct healing. Topical antimicrobials are the most common first-line self-medications; however, treatment failure is not uncommon and emerging resistance to antibiotics is alarming. Chlorquinaldol is an antimicrobial with a wide spectrum of activity and desirable characteristics for topical application. Aim of this study was to evaluate the efficacy of chlorquinaldol to prevent or eradicate S. aureus and P. aeruginosa biofilms, in comparison to classic topical antibiotics like gentamicin and fusidic acid.Methods: Minimum inhibitory concentrations (MIC) were assessed for each strain and subinhibitory concentrations (1/2 and 1/4 MIC) were used in the biofilm assay. Antimicrobial assays were performed during biofilm formation or were applied on mature biofilms and were evaluated by means of crystal violet assay and confocal laser scan microscopy.Results: Chlorquinaldol and gentamicin were the most effective antimicrobials in both eradicating and preventing pathogens biofilm; however, resistance to methicillin and impermeability to carbapenems impaired chlorquinaldol effect. In addition, similarly to other hydroxyquinolines, aspecific metal chelation is here proposed as chlorquinaldol mode of action.Conclusion: Relying on an acceptable antibiofilm and a wide spectrum of activity, an aspecific mode of action and consequent absence of resistance development, chlorquinaldol proved to be a good antimicrobial for topical use.
No
English
Pseudomonas aeruginosa; Staphylococcus aureus; biofilm; chlorquinaldol; skin and wound tissue infection
Settore MED/07 - Microbiologia e Microbiologia Clinica
Articolo
Esperti anonimi
Pubblicazione scientifica
2019
DOVE MEDICAL PRESS LTD
12
2177
2189
13
Pubblicato
Periodico con rilevanza internazionale
orcid
pubmed
crossref
wos
datacite
Aderisco
info:eu-repo/semantics/article
Chlorquinaldol, a topical agent for skin and wound infections: anti-biofilm activity and biofilm-related antimicrobial cross-resistance / A. Bidossi, M. Bottagisio, R. De Grandi, L. Drago, E. DE VECCHI. - In: INFECTION AND DRUG RESISTANCE. - ISSN 1178-6973. - 12:(2019), pp. 2177-2189. [10.2147/IDR.S211007]
open
Prodotti della ricerca::01 - Articolo su periodico
5
262
Article (author)
Periodico con Impact Factor
A. Bidossi, M. Bottagisio, R. De Grandi, L. Drago, E. DE VECCHI
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/970612
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