Immunohistochemical Distribution of Serotonin Transporter (SERT) in the Optic Lobe of the Honeybee, Apis mellifera

Simple Summary Serotonin is ubiquitously expressed in vertebrates and invertebrates, where it regulates specific behavioural patterns. Though the specific effects of serotonin release in the optic lobe are not entirely known, increasing evidence associates the serotonergic system with optic lobe-mediated behaviours. In this study, the localization of serotonin transporter (SERT) was immunohistochemically analysed in the optic lobes of moderate, docile and aggressive worker honeybees. SERT-immunoreactive fibres were stratified in the optic lobe and distributed in the three visual neuropils: lamina, medulla and lobula. Interestingly, SERT immunoreactivity was inversely related to aggressive behaviour. The present study indicates that low levels of serotonin in the optic lobe are associated with aggressive behaviour. Visual information is processed in the optic lobes, which consist of three retinotopic neuropils. These are the lamina, the medulla and the lobula. Biogenic amines play a crucial role in the control of insect responsiveness, and serotonin is clearly related to aggressiveness in invertebrates. Previous studies suggest that serotonin modulates aggression-related behaviours, possibly via alterations in optic lobe activity. The aim of this investigation was to immunohistochemically localize the distribution of serotonin transporter (SERT) in the optic lobe of moderate, docile and aggressive worker honeybees. SERT-immunoreactive fibres showed a wide distribution in the lamina, medulla and lobula; interestingly, the highest percentage of SERT immunoreactivity was observed across all the visual neuropils of the docile group. Although future research is needed to determine the relationship between the distribution of serotonin fibres in the honeybee brain and aggressive behaviours, our immunohistochemical study provides an anatomical basis supporting the role of serotonin in aggressive behaviour in the honeybee.