The toxicity of particulate matter (PM) is strictly associated with its physical-chemical characteristics, such as size or chemical composition. While these properties depend on the origin of the particles, the study of the toxicological profile of PM from single sources has rarely been highlighted. Hence, the focus of this research was to investigate the biological effects of PM from five relevant sources of atmospheric PM: diesel exhaust particles, coke dust, pellet ashes, incinerator ashes, and brake dust. Cytotoxicity, genotoxicity, oxidative, and inflammatory response were assessed in a bronchial cell line (BEAS-2B). BEAS-2B cells were exposed to different concentrations (25, 50, 100, and 150 μg/mL medium) of particles suspended in water. The exposure lasted 24 h for all the assays performed, except for reactive oxygen species, which were evaluated after 30 min, 1 h, and 4 h of treatment. The results showed a different action of the five types of PM. All the tested samples showed a genotoxic action on BEAS-2B, even in the absence of oxidative stress induction. Pellet ashes seemed to be the only ones able to induce oxidative stress by boosting the formation of reactive oxygen species, while brake dust resulted in the most cytotoxic. In conclusion, the study elucidated the differential response of bronchial cells to PM samples generated by different sources. The comparison could be a starting point for a regulatory intervention since it highlighted the toxic potential of each type of PM tested.

Toxicological Profile of PM from Different Sources in the Bronchial Epithelial Cell Line BEAS-2B / G. Melzi, E. Nozza, M.A. Frezzini, S. Canepari, R. Vecchi, L. Cremonesi, M. Potenza, M. Marinovich, E. Corsini. - In: TOXICS. - ISSN 2305-6304. - 11:5(2023), pp. 413.1-413.13. [10.3390/toxics11050413]

Toxicological Profile of PM from Different Sources in the Bronchial Epithelial Cell Line BEAS-2B

G. Melzi
Primo
;
E. Nozza
Secondo
;
R. Vecchi
;
L. Cremonesi;M. Potenza;M. Marinovich
Penultimo
;
E. Corsini
Ultimo
2023

Abstract

The toxicity of particulate matter (PM) is strictly associated with its physical-chemical characteristics, such as size or chemical composition. While these properties depend on the origin of the particles, the study of the toxicological profile of PM from single sources has rarely been highlighted. Hence, the focus of this research was to investigate the biological effects of PM from five relevant sources of atmospheric PM: diesel exhaust particles, coke dust, pellet ashes, incinerator ashes, and brake dust. Cytotoxicity, genotoxicity, oxidative, and inflammatory response were assessed in a bronchial cell line (BEAS-2B). BEAS-2B cells were exposed to different concentrations (25, 50, 100, and 150 μg/mL medium) of particles suspended in water. The exposure lasted 24 h for all the assays performed, except for reactive oxygen species, which were evaluated after 30 min, 1 h, and 4 h of treatment. The results showed a different action of the five types of PM. All the tested samples showed a genotoxic action on BEAS-2B, even in the absence of oxidative stress induction. Pellet ashes seemed to be the only ones able to induce oxidative stress by boosting the formation of reactive oxygen species, while brake dust resulted in the most cytotoxic. In conclusion, the study elucidated the differential response of bronchial cells to PM samples generated by different sources. The comparison could be a starting point for a regulatory intervention since it highlighted the toxic potential of each type of PM tested.
diesel exhaust particle; coke dust; incinerator ash; pellet ash; brake dust; genotoxicity; oxidative stress; BEAS-2B; in vitro
Settore BIO/14 - Farmacologia
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
Settore CHIM/12 - Chimica dell'Ambiente e dei Beni Culturali
Settore FIS/03 - Fisica della Materia
   Redox-activity and Health-effects of Atmospheric Primary and Secondary aerosol (RHAPS)
   RHAPS
   MINISTERO DELL'ISTRUZIONE E DEL MERITO
   2017MSN7M8_002
2023
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/967307
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