Mutations in PCDH19 gene, which encodes protocadherin-19 (PCDH19), cause Developmental and Epileptic Encephalopathy 9 (DEE9). Heterogeneous loss of PCDH19 expression in neurons is considered a key determinant of the disorder; however, how PCDH19 mosaic expression affects neuronal network activity and circuits is largely unclear. Here, we show that the hippocampus of Pcdh19 mosaic mice is characterized by structural and functional synaptic defects and by the presence of PCDH19-negative hyperexcitable neurons. Furthermore, global reduction of network firing rate and increased neuronal synchronization have been observed in different limbic system areas. Finally, network activity analysis in freely behaving mice revealed a decrease in excitatory/inhibitory ratio and functional hyperconnectivity within the limbic system of Pcdh19 mosaic mice. Altogether, these results indicate that altered PCDH19 expression profoundly affects circuit wiring and functioning, and provide new key to interpret DEE9 pathogenesis.

Neuronal network activity and connectivity are impaired in a conditional knockout mouse model with PCDH19 mosaic expression / G. Giansante, S. Mazzoleni, A.G. Zippo, L. Ponzoni, A. Ghilardi, G. Maiellano, E. Lewerissa, E. van Hugte, N. Nadif Kasri, M. Francolini, M. Sala, L. Murru, S. Bassani, M. Passafaro. - In: MOLECULAR PSYCHIATRY. - ISSN 1359-4184. - (2023), pp. 1-16. [Epub ahead of print] [10.1038/s41380-023-02022-1]

Neuronal network activity and connectivity are impaired in a conditional knockout mouse model with PCDH19 mosaic expression

S. Mazzoleni
Secondo
;
A.G. Zippo;L. Ponzoni;A. Ghilardi;G. Maiellano;M. Francolini;M. Sala;S. Bassani
Penultimo
;
2023

Abstract

Mutations in PCDH19 gene, which encodes protocadherin-19 (PCDH19), cause Developmental and Epileptic Encephalopathy 9 (DEE9). Heterogeneous loss of PCDH19 expression in neurons is considered a key determinant of the disorder; however, how PCDH19 mosaic expression affects neuronal network activity and circuits is largely unclear. Here, we show that the hippocampus of Pcdh19 mosaic mice is characterized by structural and functional synaptic defects and by the presence of PCDH19-negative hyperexcitable neurons. Furthermore, global reduction of network firing rate and increased neuronal synchronization have been observed in different limbic system areas. Finally, network activity analysis in freely behaving mice revealed a decrease in excitatory/inhibitory ratio and functional hyperconnectivity within the limbic system of Pcdh19 mosaic mice. Altogether, these results indicate that altered PCDH19 expression profoundly affects circuit wiring and functioning, and provide new key to interpret DEE9 pathogenesis.
Settore BIO/13 - Biologia Applicata
   A multidisciplinary approach to study protocadherin-19: from neuronal function to the “cellular interference” pathogenic mechanism
   MINISTERO DELL'ISTRUZIONE E DEL MERITO
   20172C9HLW_002
2023
30-mar-2023
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/962616
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