We developed a polymerase reaction free, low-cost and sensitive assay for the colorimetric detection of Human Papilloma Virus (HPV), based on the use of a smart design exploiting magnetic microbeads, chimeric RNA/DNAzyme oligonucleotides, and double signal amplification. This method allows obtaining a fast response with a detection limit of 10 pM, avoiding the amplification of the target via traditional PCR.
Colorimetric detection of human papilloma virus by double isothermal amplification / S. Persano, P. Valentini, J.H. Kim, P.P. Pompa. - In: CHEMICAL COMMUNICATIONS. - ISSN 1359-7345. - 49:90(2013 Oct 17), pp. 10605-10607. [10.1039/c3cc45459b]
Colorimetric detection of human papilloma virus by double isothermal amplification
S. PersanoPrimo
;
2013
Abstract
We developed a polymerase reaction free, low-cost and sensitive assay for the colorimetric detection of Human Papilloma Virus (HPV), based on the use of a smart design exploiting magnetic microbeads, chimeric RNA/DNAzyme oligonucleotides, and double signal amplification. This method allows obtaining a fast response with a detection limit of 10 pM, avoiding the amplification of the target via traditional PCR.
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