We describe a new method for the detection of miRNA in biological samples. This technology is based on the isothermal nicking enzyme amplification reaction and subsequent hybridization of the amplification product with gold nanoparticles and magnetic microparticles (barcode system) to achieve naked-eye colorimetric detection. This platform was used to detect a specific miRNA (miRNA-10b) associated with breast cancer, and attomolar sensitivity was demonstrated. The assay was validated in cell culture lysates from breast cancer cells and in serum from a mouse model of breast cancer.
Label-Free Isothermal Amplification Assay for Specific and Highly Sensitive Colorimetric miRNA Detection / S. Persano, M.L. Guevara, J. Wolfram, E. Blanco, H. Shen, M. Ferrari, P.P. Pompa. - In: ACS OMEGA. - ISSN 2470-1343. - 1:3(2016), pp. 448-455. [10.1021/acsomega.6b00109]
Label-Free Isothermal Amplification Assay for Specific and Highly Sensitive Colorimetric miRNA Detection
S. Persano;
2016
Abstract
We describe a new method for the detection of miRNA in biological samples. This technology is based on the isothermal nicking enzyme amplification reaction and subsequent hybridization of the amplification product with gold nanoparticles and magnetic microparticles (barcode system) to achieve naked-eye colorimetric detection. This platform was used to detect a specific miRNA (miRNA-10b) associated with breast cancer, and attomolar sensitivity was demonstrated. The assay was validated in cell culture lysates from breast cancer cells and in serum from a mouse model of breast cancer.
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
