The dark fermentation of lignocellulose hydrolysates is a promising process for the pro- duction of hydrogen from renewable sources. Nevertheless, hydrogen yields are often lower than those obtained from other carbohydrate sources due to the presence of microbial growth inhibitors in lignocellulose hydrolysates. In this study, a microbial consortium for the production of hydrogen by dark fermentation has been obtained from a wild methanogenic sludge by means of thermal treatments. The consortium has been initially acclimated to a glucose-based medium and then used as inoculum for the fermentation of Arundo donax hydrolysates. Hydrogen yields obtained from fermentation of A. donax hydrolysates were lower than those obtained from glucose fermentation using the same inoculum (0.30 ± 0.05 versus 1.11 ± 0.06 mol of H2 per mol of glucose equivalents). The hydrogen-producing bacteria belonged mainly to the Enterobacteriaceae family in cultures growing on glucose and to Clostridium in those growing on A. donax hydrolysate. In the latter cultures, Lacto- bacillus outcompeted Enterobacteriaceae, although Clostridium also increased. Lactobacillus outgrowth could account for the lower yields observed in cultures growing on A. donax hydrolysate.

Dark Fermentation of Arundo donax: Characterization of the Anaerobic Microbial Consortium / G. Toscano, G. Zuccaro, A. Corsini, S. Zecchin, L. Cavalca. - In: ENERGIES. - ISSN 1996-1073. - 16:4(2023 Feb 11), pp. 1813.1-1813.16. [10.3390/en16041813]

Dark Fermentation of Arundo donax: Characterization of the Anaerobic Microbial Consortium

A. Corsini
Formal Analysis
;
S. Zecchin
Penultimo
Formal Analysis
;
L. Cavalca
Ultimo
Conceptualization
2023

Abstract

The dark fermentation of lignocellulose hydrolysates is a promising process for the pro- duction of hydrogen from renewable sources. Nevertheless, hydrogen yields are often lower than those obtained from other carbohydrate sources due to the presence of microbial growth inhibitors in lignocellulose hydrolysates. In this study, a microbial consortium for the production of hydrogen by dark fermentation has been obtained from a wild methanogenic sludge by means of thermal treatments. The consortium has been initially acclimated to a glucose-based medium and then used as inoculum for the fermentation of Arundo donax hydrolysates. Hydrogen yields obtained from fermentation of A. donax hydrolysates were lower than those obtained from glucose fermentation using the same inoculum (0.30 ± 0.05 versus 1.11 ± 0.06 mol of H2 per mol of glucose equivalents). The hydrogen-producing bacteria belonged mainly to the Enterobacteriaceae family in cultures growing on glucose and to Clostridium in those growing on A. donax hydrolysate. In the latter cultures, Lacto- bacillus outcompeted Enterobacteriaceae, although Clostridium also increased. Lactobacillus outgrowth could account for the lower yields observed in cultures growing on A. donax hydrolysate.
biohydrogen; dark fermentation; Arundo donax; lignocellulosic biomass; anaerobic consortia; Clostridium; Klebsiella; Enterobacter; lactic acid bacteria
Settore AGR/16 - Microbiologia Agraria
11-feb-2023
Article (author)
File in questo prodotto:
File Dimensione Formato  
energies-16-01813.pdf

accesso aperto

Descrizione: Article
Tipologia: Publisher's version/PDF
Dimensione 1.68 MB
Formato Adobe PDF
1.68 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/957107
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 0
  • ???jsp.display-item.citation.isi??? 0
social impact