Objective: Bacteria identification inside the dental tissue is a complex procedure requiring specific protocols. This study aimed to compare two classical Gram staining methods with a new staining method proposed by the authors to detect Gram-positive and Gram-negative bacteria in dental histological samples of human dentin. Methods: Ten human teeth, extracted because of various pathologies, were decalcified, dehydrated, and paraffin-embedded. Then, approximately 100 serial sections of 4 µm thickness were made per sample. The serial sections were placed on glass slides and were stained according to Brown-Brenn, Brown-Hopps, and a proposed modification of Brown-Brenn staining. Both ATCC strains, smeared on glass slides, were stained following each method's instructions used in histological samples. Results: From a qualitative evaluation, the Brown-Brenn method resulted in better staining of Gram-positive bacteria, while the authors' proposed staining technique was more oriented towards Gram-negative bacteria. On the other hand, the Brown-Hopps showed quite a balance in detecting Gram-positive and Gram-negative bacteria. Unlike the Brown-Brenn stain, the other two protocols showed better stainability of Gram-negative microorganisms in bacterial-smeared samples. Conclusion: All staining techniques evaluated in this article can identify bacteria, but the outcome can change according to the staining procedure used.
A simplified method for detecting Gram-positive and Gram-negative bacteria in dental histological samples: A preliminary and comparative study / P. Savadori, S. Dalfino, M. Piazzoni, M. Parrini, M. Del Fabbro, G.M. Tartaglia, L. Giardino. - In: ACTA HISTOCHEMICA. - ISSN 0065-1281. - 125:1(2023 Jan), pp. 151992.1-151992.7. [10.1016/j.acthis.2022.151992]
A simplified method for detecting Gram-positive and Gram-negative bacteria in dental histological samples: A preliminary and comparative study
P. SavadoriPrimo
;S. Dalfino
Secondo
;M. Parrini;M. Del Fabbro;G.M. TartagliaPenultimo
;L. GiardinoUltimo
2023
Abstract
Objective: Bacteria identification inside the dental tissue is a complex procedure requiring specific protocols. This study aimed to compare two classical Gram staining methods with a new staining method proposed by the authors to detect Gram-positive and Gram-negative bacteria in dental histological samples of human dentin. Methods: Ten human teeth, extracted because of various pathologies, were decalcified, dehydrated, and paraffin-embedded. Then, approximately 100 serial sections of 4 µm thickness were made per sample. The serial sections were placed on glass slides and were stained according to Brown-Brenn, Brown-Hopps, and a proposed modification of Brown-Brenn staining. Both ATCC strains, smeared on glass slides, were stained following each method's instructions used in histological samples. Results: From a qualitative evaluation, the Brown-Brenn method resulted in better staining of Gram-positive bacteria, while the authors' proposed staining technique was more oriented towards Gram-negative bacteria. On the other hand, the Brown-Hopps showed quite a balance in detecting Gram-positive and Gram-negative bacteria. Unlike the Brown-Brenn stain, the other two protocols showed better stainability of Gram-negative microorganisms in bacterial-smeared samples. Conclusion: All staining techniques evaluated in this article can identify bacteria, but the outcome can change according to the staining procedure used.File | Dimensione | Formato | |
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