PURPOSE: To objectively grade posterior segment in-flammation by measuring vitreous cells and haze on opti-cal coherence tomography (OCT) scans and to compare OCT-based results with clinical grading. center dot DESIGN: Evaluation of a diagnostic test.METHODS: OCT scans of patients with uveitis were collected at 3 timepoints: with active (T0), clinically im-proving (T1), and resolved (T2) inflammation. At each visit, visual acuity and clinical grading of the vitreous haze (National Eye Institute [NEI] scale) were assessed. The density of vitreous cells was calculated on each OCT scan manually and automatically through a bespoke algo-rithm. Vitreous haze was indirectly measured on OCT scans by calculating the vitreous/retinal pigmented ep-ithelium (VIT/RPE)-relative intensity manually and au-tomatically. The variation of OCT-derived measurements over time was assessed. OCT-derived measurements were compared with clinical grading.RESULTS: A total of 222 scans from 74 eyes were an-alyzed. Both vitreous cell density and VIT/RPE-relative intensity significantly decreased over time. Cell density correlated with the clinical grading with a significant increase at each grade of the NEI scale. By contrast, the VIT/RPE-relative intensity was positively correlated with the clinical grade overall but there was no significant difference when comparing contiguous grades of the NEI scale. Infectious uveitis had a higher cell density. The in-traclass correlation coefficient between manual and auto-matic assessment was 0.83 for cell density and 0.423 for the VIT/RPE-relative intensity. CONCLUSIONS: Posterior segment inflammation could be objectively graded through OCT scans. Vitreous cell density was assessed manually and automatically with good agreement and correlated better with NEI clini-cal grading compared with VIT/RPE-relative intensity.

Objective Quantification of Posterior Segment Inflammation: Measuring Vitreous Cells and Haze Using Optical Coherence Tomography / F. Zicarelli, G. Ometto, G. Montesano, S. Motta, L. De Simone, L. Cimino, G. Staurenghi, A. Agarwal, F. Pichi, A. Invernizzi. - In: AMERICAN JOURNAL OF OPHTHALMOLOGY. - ISSN 0002-9394. - 245:(2023 Jan), pp. 134-144. [10.1016/j.ajo.2022.08.025]

Objective Quantification of Posterior Segment Inflammation: Measuring Vitreous Cells and Haze Using Optical Coherence Tomography

G. Staurenghi;A. Invernizzi
Ultimo
2023

Abstract

PURPOSE: To objectively grade posterior segment in-flammation by measuring vitreous cells and haze on opti-cal coherence tomography (OCT) scans and to compare OCT-based results with clinical grading. center dot DESIGN: Evaluation of a diagnostic test.METHODS: OCT scans of patients with uveitis were collected at 3 timepoints: with active (T0), clinically im-proving (T1), and resolved (T2) inflammation. At each visit, visual acuity and clinical grading of the vitreous haze (National Eye Institute [NEI] scale) were assessed. The density of vitreous cells was calculated on each OCT scan manually and automatically through a bespoke algo-rithm. Vitreous haze was indirectly measured on OCT scans by calculating the vitreous/retinal pigmented ep-ithelium (VIT/RPE)-relative intensity manually and au-tomatically. The variation of OCT-derived measurements over time was assessed. OCT-derived measurements were compared with clinical grading.RESULTS: A total of 222 scans from 74 eyes were an-alyzed. Both vitreous cell density and VIT/RPE-relative intensity significantly decreased over time. Cell density correlated with the clinical grading with a significant increase at each grade of the NEI scale. By contrast, the VIT/RPE-relative intensity was positively correlated with the clinical grade overall but there was no significant difference when comparing contiguous grades of the NEI scale. Infectious uveitis had a higher cell density. The in-traclass correlation coefficient between manual and auto-matic assessment was 0.83 for cell density and 0.423 for the VIT/RPE-relative intensity. CONCLUSIONS: Posterior segment inflammation could be objectively graded through OCT scans. Vitreous cell density was assessed manually and automatically with good agreement and correlated better with NEI clini-cal grading compared with VIT/RPE-relative intensity.
Settore MED/30 - Malattie Apparato Visivo
7-set-2022
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/951812
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