We have previously demonstrated that RACK1, which expression is under steroid hormone control, plays an important role in the activation of immune cells and its expression can be useful to evaluate the immunotoxic profile of endocrine disrupting chemicals (EDCs). Hence, we investigated the effects of three contaminating and persistent pesticides: the fungicide vinclozolin (VIN), the herbicide atrazine (ATR) and the insecticide cyper-methrin (CYP) on RACK1 expression and on innate immune response. VIN resulted in modest alteration of RACK1 while ATR and CYP reduced in a dose dependent manner RACK1 expression, ultimately leading to the decrease in lipopolysaccharide-induced IL-8 and TNF-alpha release and CD86 and CD54 surface marker expression. Moreover, our data indicate that, after exposure to EDCs, alterations of RACK1 expression can also occur with mechanisms not directly mediated by an interaction with a nuclear or membrane steroid receptors. Therefore, RACK1 could represent a useful EDCs screening tool to evaluate their immunotoxic potential and to dissect their mechanisms of action.

Effects of endocrine active contaminating pesticides on RACK1 expression and immunological consequences in THP-1 cells / A. Maddalon, M. Masi, M. Iulini, P. Linciano, V. Galbiati, M. Marinovich, M. Racchi, E. Buoso, E. Corsini. - In: ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY. - ISSN 1382-6689. - 95:(2022 Oct), pp. 103971.1-103971.13. [10.1016/j.etap.2022.103971]

Effects of endocrine active contaminating pesticides on RACK1 expression and immunological consequences in THP-1 cells

A. Maddalon
Primo
;
M. Iulini;V. Galbiati;M. Marinovich;E. Corsini
Ultimo
2022

Abstract

We have previously demonstrated that RACK1, which expression is under steroid hormone control, plays an important role in the activation of immune cells and its expression can be useful to evaluate the immunotoxic profile of endocrine disrupting chemicals (EDCs). Hence, we investigated the effects of three contaminating and persistent pesticides: the fungicide vinclozolin (VIN), the herbicide atrazine (ATR) and the insecticide cyper-methrin (CYP) on RACK1 expression and on innate immune response. VIN resulted in modest alteration of RACK1 while ATR and CYP reduced in a dose dependent manner RACK1 expression, ultimately leading to the decrease in lipopolysaccharide-induced IL-8 and TNF-alpha release and CD86 and CD54 surface marker expression. Moreover, our data indicate that, after exposure to EDCs, alterations of RACK1 expression can also occur with mechanisms not directly mediated by an interaction with a nuclear or membrane steroid receptors. Therefore, RACK1 could represent a useful EDCs screening tool to evaluate their immunotoxic potential and to dissect their mechanisms of action.
atrazine; cypermethrin; Endocrine disrupting chemicals (EDCs); immunotoxicity; RACK1; vinclozolin; hormones; humans; interleukin-8; lipopolysaccharides; neoplasm proteins; receptors for activated C Kinase; THP-1 cells; tumor necrosis factor-alpha; atrazine; endocrine disruptors; fungicides, industrial; herbicides; insecticides; pesticides
Settore BIO/14 - Farmacologia
   ENDOCRINE DISRUPTORS: INVESTIGATION OF THE EFFECTS ON THE IMMUNE AND NERVOUS SYSTEMS (EDoNIS)
   EDoNIS
   MINISTERO DELL'ISTRUZIONE E DEL MERITO
   2017MLC3NF_001

   Study of the crosstalk between multiple pathways in the regulation of inflammatory processes in models of chronic and degenerative diseases
   MINISTERO DELL'ISTRUZIONE E DEL MERITO
   2017B9NCSX_004
ott-2022
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/947296
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