In the past four decades, the bovine model has been highly informative and inspiring to assisted reproductive technologies (ART) in other species. Most of the recent advances in ART have come from studies in cattle, particularly those unveiling the importance of several processes that must be recapitulated in vitro to ensure the proper development of the oocyte. The maintenance of structural and functional communications between the cumulus cells and the oocyte and a well-orchestrated chromatin remodeling with the gradual silencing of transcriptional activity represent essential processes for the progressive acquisition of oocyte developmental competence. These markers are now considered the milestones of physiological approaches to increase the efficiency of reproductive technologies. Different in vitro approaches have been proposed. In particular, the so-called "pre-IVM" or "prematuration" is a culture step performed before in vitro maturation (IVM) to support the completion of the oocyte differentiation process. Although these attempts only partially improved the embryo quality and yield, they currently represent a proof of principle that oocytes retrieved from an ovary or an ovarian batch shouldn't be treated as a whole and that tailored approaches can be developed for culturing competent oocytes in several species, including humans. An advancement in ART's efficiency would be desirable in carnivores, where the success is still limited. Since the progress in reproductive medicine has often come from comparative studies, this review highlights aspects that have been critical in other species and how they may be extended to carnivores.

Progress toward species-tailored prematuration approaches in carnivores / A.M. Luciano, F. Franciosi, P. Dey, M. Ladron De Guevara, N. Monferini, S.K.N. Bonumallu, G. Musmeci, F. Fagali Franchi, R. Garcia Barros, M. Colombo, V. Lodde. - In: THERIOGENOLOGY. - ISSN 0093-691X. - 196:(2023), pp. 202-213. ((Intervento presentato al 24. convegno International Symposium on Canine and Feline Reproduction in a joint meeting with the European Veterinary Society for Small Animal Reproduction Congress tenutosi a Milano nel 2022 [10.1016/j.theriogenology.2022.11.016].

Progress toward species-tailored prematuration approaches in carnivores

A.M. Luciano
Primo
;
F. Franciosi
Secondo
;
P. Dey;M. Ladron De Guevara;N. Monferini;S.K.N. Bonumallu;G. Musmeci;F. Fagali Franchi;R. Garcia Barros;M. Colombo
Penultimo
;
V. Lodde
Ultimo
2023

Abstract

In the past four decades, the bovine model has been highly informative and inspiring to assisted reproductive technologies (ART) in other species. Most of the recent advances in ART have come from studies in cattle, particularly those unveiling the importance of several processes that must be recapitulated in vitro to ensure the proper development of the oocyte. The maintenance of structural and functional communications between the cumulus cells and the oocyte and a well-orchestrated chromatin remodeling with the gradual silencing of transcriptional activity represent essential processes for the progressive acquisition of oocyte developmental competence. These markers are now considered the milestones of physiological approaches to increase the efficiency of reproductive technologies. Different in vitro approaches have been proposed. In particular, the so-called "pre-IVM" or "prematuration" is a culture step performed before in vitro maturation (IVM) to support the completion of the oocyte differentiation process. Although these attempts only partially improved the embryo quality and yield, they currently represent a proof of principle that oocytes retrieved from an ovary or an ovarian batch shouldn't be treated as a whole and that tailored approaches can be developed for culturing competent oocytes in several species, including humans. An advancement in ART's efficiency would be desirable in carnivores, where the success is still limited. Since the progress in reproductive medicine has often come from comparative studies, this review highlights aspects that have been critical in other species and how they may be extended to carnivores.
Assisted reproduction; Canine; Cat; Developmental competence; Dog; Embryo; Feline; Gap junctions; Large scale chromatin configuration; Meiosis; Meiotic arrest; Oocyte; Pre-IVM; Prematuration
Settore VET/01 - Anatomia degli Animali Domestici
Settore VET/10 - Clinica Ostetrica e Ginecologia Veterinaria
European Veterinary Society for Small Animal Reproduction
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/947272
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