Background: Many studies consider the contamination of dental unit waterlines (DUWLs), but few of them have studied the possible presence of antibiotic resistant Pseudomonas aeruginosa in the DUWLs. Aims: Investigation of the presence of P. aeruginosa and Pseudomonas spp. strains in DUWLs and evaluation of their resistance to six antibiotics (ceftazidime, netilmicin, piperacillin/tazobactam, meropenem, levofloxacin, colistin sulfate) at a public dental clinic in Milan, Italy. Results: Dental units were contaminated by P. aeruginosa with loads of 2–1,000 CFU/L and were mainly located on the mezzanine floor, with a range of 46–54%, while Pseudomonas spp. were primarily found on the first and second floors, ranging from 50 to 91%. P. aeruginosa was antibiotic resistant in 30% of the strains tested, andPseudomonas spp. in 31.8%. Cold water from controls was also contaminated by these microorganisms. Conclusion: Monitoring antibiotic resistance in the water and adopting disinfection procedures on DUs are suggested within the Water Safety Plan.

Dental unit water content and antibiotic resistance of Pseudomonas aeruginosa and Pseudomonas species: a case study / M. Tesauro, M. Consonni, I. Grappasonni, G. Lodi, R. Mattina. - In: JOURNAL OF ORAL MICROBIOLOGY. - ISSN 2000-2297. - 14:1(2022), pp. 2107316.1-2107316.8. [10.1080/20002297.2022.2107316]

Dental unit water content and antibiotic resistance of Pseudomonas aeruginosa and Pseudomonas species: a case study

M. Tesauro
Primo
;
M. Consonni
Secondo
;
G. Lodi
Penultimo
;
R. Mattina
Ultimo
2022

Abstract

Background: Many studies consider the contamination of dental unit waterlines (DUWLs), but few of them have studied the possible presence of antibiotic resistant Pseudomonas aeruginosa in the DUWLs. Aims: Investigation of the presence of P. aeruginosa and Pseudomonas spp. strains in DUWLs and evaluation of their resistance to six antibiotics (ceftazidime, netilmicin, piperacillin/tazobactam, meropenem, levofloxacin, colistin sulfate) at a public dental clinic in Milan, Italy. Results: Dental units were contaminated by P. aeruginosa with loads of 2–1,000 CFU/L and were mainly located on the mezzanine floor, with a range of 46–54%, while Pseudomonas spp. were primarily found on the first and second floors, ranging from 50 to 91%. P. aeruginosa was antibiotic resistant in 30% of the strains tested, andPseudomonas spp. in 31.8%. Cold water from controls was also contaminated by these microorganisms. Conclusion: Monitoring antibiotic resistance in the water and adopting disinfection procedures on DUs are suggested within the Water Safety Plan.
antibiotic resistance; Dental unit water line; Pseudomonas aeruginosa; Pseudomonasspp; water microbiology; water safety plan
Settore MED/42 - Igiene Generale e Applicata
Settore MED/07 - Microbiologia e Microbiologia Clinica
Settore MED/28 - Malattie Odontostomatologiche
PSRL621PRISO_01 - One Health Action Hub: task force di Ateneo per la resilienza di ecosistemi territoriali (1H_Hub) (Linea Strategica 3, Tema One health, one earth) - RISO, PATRIZIA - PSR_LINEA6_ / Piano di sviluppo di ricerca - Grandi Sfide di Ateneo - Linea 6 - 2021
PSRL218MGALL_01 - Piano di Sostegno alla Ricerca 2015-2017 - Linea 2 "Dotazione annuale per attività istituzionali" (anno 2018) - GALLI, MASSIMO - PSR_LINEA2_ / Piano di sviluppo di ricerca - Dotazioni dipartimentali - Linea 2 - 2018
23-ago-2022
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/947055
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