Structural synaptic plasticity may underlie experience and learning-dependent changes in cortical circuits. In contrast to excitatory pyramidal neurons, insight into the structural plasticity of inhibitory neurons remains limited. Interneurons are divided into various subclasses, each with specialized functions in cortical circuits. Further knowledge of subclass-specific structural plasticity of interneurons is crucial to gaining a complete mechanistic understanding of their contribution to cortical plasticity overall. Here, we describe a subpopulation of superficial cortical multipolar interneurons expressing vasoactive intestinal peptide (VIP) with high spine densities on their dendrites located in layer (L) 1, and with the electrophysiological characteristics of bursting cells. Using longitudinal imaging in vivo, we found that the majority of the spines are highly dynamic, displaying lifetimes considerably shorter than that of spines on pyramidal neurons. Using correlative light and electron microscopy, we confirmed that these VIP spines are sites of excitatory synaptic contacts, and are morphologically distinct from other spines in L1.Advanced light and electron microscopy identify a subset of cortical multipolar vasoactive intestinal peptide interneurons with high spine dynamics and characteristics that are greatly different from pyramidal neurons.

A subpopulation of cortical VIP-expressing interneurons with highly dynamic spinesIn: COMMUNICATIONS BIOLOGY. - ISSN 2399-3642. - 5:1(2022), pp. 352.1-352.15. [10.1038/s42003-022-03278-z]

A subpopulation of cortical VIP-expressing interneurons with highly dynamic spines

F. Brandalise;
2022

Abstract

Structural synaptic plasticity may underlie experience and learning-dependent changes in cortical circuits. In contrast to excitatory pyramidal neurons, insight into the structural plasticity of inhibitory neurons remains limited. Interneurons are divided into various subclasses, each with specialized functions in cortical circuits. Further knowledge of subclass-specific structural plasticity of interneurons is crucial to gaining a complete mechanistic understanding of their contribution to cortical plasticity overall. Here, we describe a subpopulation of superficial cortical multipolar interneurons expressing vasoactive intestinal peptide (VIP) with high spine densities on their dendrites located in layer (L) 1, and with the electrophysiological characteristics of bursting cells. Using longitudinal imaging in vivo, we found that the majority of the spines are highly dynamic, displaying lifetimes considerably shorter than that of spines on pyramidal neurons. Using correlative light and electron microscopy, we confirmed that these VIP spines are sites of excitatory synaptic contacts, and are morphologically distinct from other spines in L1.Advanced light and electron microscopy identify a subset of cortical multipolar vasoactive intestinal peptide interneurons with high spine dynamics and characteristics that are greatly different from pyramidal neurons.
Neuronal Plasticity; Neurons; Pyramidal Cells; Interneurons; Vasoactive Intestinal Peptide
Settore BIO/09 - Fisiologia
2022
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/946709
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