Fluorescence lifetime imaging microscopy–fluorescence resonance energy transfer (FLIM–FRET) technique is a novel tool to study protein–protein interaction combining FRET detection, lifetime measurements, and high-resolution imaging. This chapter describes a FLIM–FRET protocol for the detection and quantification of heterogeneous huntingtin aggregates in HeLa cells co-transfected with huntingtin variants containing polyglutamine tracts of different lengths.

FLIM-FRET investigation of heterogeneous huntingtin aggregation in HeLa cells / M.C. Lionetti, L. Caterina (METHODS IN MOLECULAR BIOLOGY). - In: Protein Aggregation / [a cura di] A.S. Cieplak. - [s.l] : Humana, 2023. - ISBN 978-1-0716-2596-5. - pp. 595-604 [10.1007/978-1-0716-2597-2_36]

FLIM-FRET investigation of heterogeneous huntingtin aggregation in HeLa cells

M.C. Lionetti
Primo
;
L. Caterina
Ultimo
2023

Abstract

Fluorescence lifetime imaging microscopy–fluorescence resonance energy transfer (FLIM–FRET) technique is a novel tool to study protein–protein interaction combining FRET detection, lifetime measurements, and high-resolution imaging. This chapter describes a FLIM–FRET protocol for the detection and quantification of heterogeneous huntingtin aggregates in HeLa cells co-transfected with huntingtin variants containing polyglutamine tracts of different lengths.
Confocal microscopy; FLIM; FRET; Huntingtin; Protein aggregation; Quantitative biology
Settore MED/04 - Patologia Generale
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/945772
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