FLIM-FRET investigation of heterogeneous huntingtin aggregation in HeLa cells

Fluorescence lifetime imaging microscopy–fluorescence resonance energy transfer (FLIM–FRET) technique is a novel tool to study protein–protein interaction combining FRET detection, lifetime measurements, and high-resolution imaging. This chapter describes a FLIM–FRET protocol for the detection and quantification of heterogeneous huntingtin aggregates in HeLa cells co-transfected with huntingtin variants containing polyglutamine tracts of different lengths.