Objectives: MicroRNAs (miRNAs) are short, endogenous noncoding RNA molecules that can bind to certain parts of target mRNAs, thereby regulating gene expression. Studies showed that miRNAs could be upor downregulated in different allergic skin conditions but there is still need for further studies. The aim of this study was to investigate the expression of miRNAs in response to the common contact allergen Bandrowski's base (BB), the principal allergen in patients reacting to p-phenylenediamine (PPD). Materials and Methods: The human promyelocytic cell line THP-1 was exposed to BB at a concentration of 1 mu g/mL for 24, 48, and 72 h. The dose was selected from the results of cytotoxicity assays. RNA was purified and miRNA expression profile and real-time polymerase chain reaction (RTPCR) were performed to identify upor downregulated miRNAs and confirm their modulations. Results: Among the different modulated miRNAs, the upregulation of miRNA-155 and the downregulation of miRNA-21 were found to be important because these are related to immune system. This expression profile of miRNAs was also confirmed by RT-PCR. Conclusion: These preliminary results showed that miR-155 and miR-21 may play a role in the pathogenesis of allergic contact dermatitis, but further studies are needed to clarify their definite roles.
Evaluation of the Possible Role of miRNAs in Chemical Allergen Potency = Mirna’ların kimyasal alerjen potansiyellerinin olası rollerinin değerlendirilmesi / H. Gül ANLAR, V. Galbiati, E. Corsini, N. Başaran. - In: TURKISH JOURNAL OF PHARMACEUTICAL SCIENCES. - ISSN 1304-530X. - 17:4(2020), pp. 452-456. [10.4274/tjps.galenos.2019.25349]
Evaluation of the Possible Role of miRNAs in Chemical Allergen Potency = Mirna’ların kimyasal alerjen potansiyellerinin olası rollerinin değerlendirilmesi
V. GalbiatiSecondo
Membro del Collaboration Group
;E. CorsiniPenultimo
Membro del Collaboration Group
;
2020
Abstract
Objectives: MicroRNAs (miRNAs) are short, endogenous noncoding RNA molecules that can bind to certain parts of target mRNAs, thereby regulating gene expression. Studies showed that miRNAs could be upor downregulated in different allergic skin conditions but there is still need for further studies. The aim of this study was to investigate the expression of miRNAs in response to the common contact allergen Bandrowski's base (BB), the principal allergen in patients reacting to p-phenylenediamine (PPD). Materials and Methods: The human promyelocytic cell line THP-1 was exposed to BB at a concentration of 1 mu g/mL for 24, 48, and 72 h. The dose was selected from the results of cytotoxicity assays. RNA was purified and miRNA expression profile and real-time polymerase chain reaction (RTPCR) were performed to identify upor downregulated miRNAs and confirm their modulations. Results: Among the different modulated miRNAs, the upregulation of miRNA-155 and the downregulation of miRNA-21 were found to be important because these are related to immune system. This expression profile of miRNAs was also confirmed by RT-PCR. Conclusion: These preliminary results showed that miR-155 and miR-21 may play a role in the pathogenesis of allergic contact dermatitis, but further studies are needed to clarify their definite roles.File | Dimensione | Formato | |
---|---|---|---|
TJPS-17-452-En.pdf
accesso aperto
Tipologia:
Publisher's version/PDF
Dimensione
496.64 kB
Formato
Adobe PDF
|
496.64 kB | Adobe PDF | Visualizza/Apri |
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.