Abstract. Glutamate synthase is a multicomponent ironsulfur flavoprotein belonging to the class of N-terminal nucleophile amidotransferases. It catalyzes the conversion of L-glutamine and 2-oxoglutarate into two molecules of L-glutamate. In recent years the X-ray structures of the ferredoxin-dependent glutamate synthase and of the a subunit of the NADPH-dependent glutamate synthase have become available. Thanks to X-ray crystallography, it is now known that the ammonia reaction intermediate is transferred via an intramolecular tunnel from the amidotransferase domain to the synthase domain over a distance of about 32 Å. Although ammonia channeling is a recurrent theme for N-terminal nucleophile and triad-type amidotransferases,the molecular mechanisms of ammonia transfer and its control are different for each known amidotransferase. This review focuses on the intriguing mechanism of action and self-regulation of glutamate synthase with a special focus on the structural data.
Glutamate synthase: a fascinating pathway from L-glutamine to L-glutamate / R..H..H. van den Heuvel, B. Curti, M.A. Vanoni, A. Mattevi. - In: CELLULAR AND MOLECULAR LIFE SCIENCES. - ISSN 1420-682X. - 61:6(2004), pp. 669-681.
Glutamate synthase: a fascinating pathway from L-glutamine to L-glutamate
B. CurtiSecondo
;M.A. VanoniPenultimo
;
2004
Abstract
Abstract. Glutamate synthase is a multicomponent ironsulfur flavoprotein belonging to the class of N-terminal nucleophile amidotransferases. It catalyzes the conversion of L-glutamine and 2-oxoglutarate into two molecules of L-glutamate. In recent years the X-ray structures of the ferredoxin-dependent glutamate synthase and of the a subunit of the NADPH-dependent glutamate synthase have become available. Thanks to X-ray crystallography, it is now known that the ammonia reaction intermediate is transferred via an intramolecular tunnel from the amidotransferase domain to the synthase domain over a distance of about 32 Å. Although ammonia channeling is a recurrent theme for N-terminal nucleophile and triad-type amidotransferases,the molecular mechanisms of ammonia transfer and its control are different for each known amidotransferase. This review focuses on the intriguing mechanism of action and self-regulation of glutamate synthase with a special focus on the structural data.Pubblicazioni consigliate
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