Anal and urethral samples from confirmed cases of monkeypox were screened for monkeypox virus (MPXV) by real-time PCR. Isolation of the virus was subsequently attempted in cell culture. Actively-replicating virus was demonstrated in 13 of 18 and 11 of 15 PCR-positive anal and urethral swabs, respectively, collected within 7 days from symptoms onset. Two asymptomatic secondary cases had detectable MPXV genetic material in urethral secretion and for one, MPXV was successfully isolated, supporting a potential MPXV sexual transmission hypothesis.
Isolation of viable monkeypox virus from anal and urethral swabs, Italy, May to July 2022 / D. Moschese, G. Pozza, D. Mileto, A. Giacomelli, M. Cutrera, M.V. Cossu, M. Matone, M. Beltrami, F. Salari, S. Antinori, A. Lombardi, G. Rizzardini. - In: EUROSURVEILLANCE. - ISSN 1560-7917. - 27:36(2022 Sep 27), pp. 2200675.1-2200675.8. [10.2807/1560-7917.ES.2022.27.36.2200675]
Isolation of viable monkeypox virus from anal and urethral swabs, Italy, May to July 2022
G. Pozza;D. Mileto;A. Giacomelli;M. Matone;M. Beltrami;F. Salari;S. Antinori;
2022
Abstract
Anal and urethral samples from confirmed cases of monkeypox were screened for monkeypox virus (MPXV) by real-time PCR. Isolation of the virus was subsequently attempted in cell culture. Actively-replicating virus was demonstrated in 13 of 18 and 11 of 15 PCR-positive anal and urethral swabs, respectively, collected within 7 days from symptoms onset. Two asymptomatic secondary cases had detectable MPXV genetic material in urethral secretion and for one, MPXV was successfully isolated, supporting a potential MPXV sexual transmission hypothesis.
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