Before the endothelial mitogenic activity of the Vascular Endothelial Growth Factor A (VEGF) was described, VEGF had already been identified for its ability to induce vascular leakage. VEGF-induced vascular leakage has been most frequently studied in vivo using the Miles assay, a simple yet invaluable technique that has allowed researchers to unravel the molecular mechanisms underpinning vascular leakage both for VEGF and other permeability inducing agents. In this protocol, a mouse is intravenously injected with Evans Blue dye before VEGF is administered locally via intradermal injection. VEGF promotes vascular leak of serum proteins in the dermis, enabling Evans Blue-labeled albumin extravasation from the circulation and subsequent accumulation in the skin. As the volume of dye extravasation is proportional to the degree of vascular leak, it can be quantified as a proxy measurement of VEGF-induced vascular leakage.

Evaluating VEGF-Induced Vascular Leakage Using the Miles Assay / J.T. Brash, C. Ruhrberg, A. Fantin (METHODS IN MOLECULAR BIOLOGY). - In: Methods in Molecular Biology / [a cura di] L. R. Fiedler, C. Pellet-Many. - Riedizione. - [s.l] : Humana Press : Springer, 2022. - ISBN 978-1-0716-2216-2. - pp. 289-295 [10.1007/978-1-0716-2217-9_21]

Evaluating VEGF-Induced Vascular Leakage Using the Miles Assay

A. Fantin
Ultimo
2022

Abstract

Before the endothelial mitogenic activity of the Vascular Endothelial Growth Factor A (VEGF) was described, VEGF had already been identified for its ability to induce vascular leakage. VEGF-induced vascular leakage has been most frequently studied in vivo using the Miles assay, a simple yet invaluable technique that has allowed researchers to unravel the molecular mechanisms underpinning vascular leakage both for VEGF and other permeability inducing agents. In this protocol, a mouse is intravenously injected with Evans Blue dye before VEGF is administered locally via intradermal injection. VEGF promotes vascular leak of serum proteins in the dermis, enabling Evans Blue-labeled albumin extravasation from the circulation and subsequent accumulation in the skin. As the volume of dye extravasation is proportional to the degree of vascular leak, it can be quantified as a proxy measurement of VEGF-induced vascular leakage.
Edema; Evans blue; Miles assay; Vascular leakage; Vascular permeability; VEGF;
Settore BIO/09 - Fisiologia
Settore BIO/13 - Biologia Applicata
Settore BIO/17 - Istologia
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/939170
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