In spermatophytes the sporophytic (diploid) and the gametophytic (haploid) generations co-exist in ovules, and the coordination of their developmental programs is of pivotal importance for plant reproduction. To achieve efficient fertilization, the haploid female gametophyte and the diploid ovule structures must coordinate their development to form a functional and correctly shaped ovule. WUSCHEL-RELATED HOMEOBOX (WOX) genes encode a family of transcription factors that share important roles in a wide range of processes throughout plant development. Here, we show that STIP is required for the correct patterning and curvature of the ovule in Arabidopsis thaliana. The knockout mutant stip-2 is characterized by a radialized ovule phenotype due to severe defects in outer integument development. In addition, alteration of STIP expression affects the correct differentiation and progression of the female germline. Finally, our results reveal that STIP is required to tightly regulate the key ovule factors INNER NO OUTER, PHABULOSA and WUSCHEL, and they define a novel genetic interplay in the regulatory networks determining ovule development.

Pivotal role of STIP in ovule pattern formation and female germline development in Arabidopsis thaliana / R. Petrella, F. Gabrieli, A. Cavalleri, K. Schneitz, L. Colombo, M. Cucinotta. - In: DEVELOPMENT. - ISSN 1477-9129. - 149:18(2022 Sep 23), pp. dev201184.1-dev201184.10. [10.1242/dev.201184]

Pivotal role of STIP in ovule pattern formation and female germline development in Arabidopsis thaliana

R. Petrella
Primo
;
F. Gabrieli
Secondo
;
A. Cavalleri;L. Colombo
Penultimo
;
M. Cucinotta
Ultimo
2022

Abstract

In spermatophytes the sporophytic (diploid) and the gametophytic (haploid) generations co-exist in ovules, and the coordination of their developmental programs is of pivotal importance for plant reproduction. To achieve efficient fertilization, the haploid female gametophyte and the diploid ovule structures must coordinate their development to form a functional and correctly shaped ovule. WUSCHEL-RELATED HOMEOBOX (WOX) genes encode a family of transcription factors that share important roles in a wide range of processes throughout plant development. Here, we show that STIP is required for the correct patterning and curvature of the ovule in Arabidopsis thaliana. The knockout mutant stip-2 is characterized by a radialized ovule phenotype due to severe defects in outer integument development. In addition, alteration of STIP expression affects the correct differentiation and progression of the female germline. Finally, our results reveal that STIP is required to tightly regulate the key ovule factors INNER NO OUTER, PHABULOSA and WUSCHEL, and they define a novel genetic interplay in the regulatory networks determining ovule development.
ovule development; plant reproduction; WOX transcription factor; ovule integuments; Arabidopsis thaliana; female gametophyte; megasporogenesis;
Settore BIO/01 - Botanica Generale
Settore BIO/18 - Genetica
Article (author)
File in questo prodotto:
File Dimensione Formato  
Preprint Petrella et al., 2022 Development.pdf

embargo fino al 23/09/2023

Tipologia: Pre-print (manoscritto inviato all'editore)
Dimensione 2.48 MB
Formato Adobe PDF
2.48 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
dev201184.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 14.6 MB
Formato Adobe PDF
14.6 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
dev201184_compressed.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 761.74 kB
Formato Adobe PDF
761.74 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/938918
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 0
  • ???jsp.display-item.citation.isi??? ND
social impact