The cell/microenvironment interface is the starting point of integrin-mediated mechanotransduction, but many details of mechanotransductive signal integration remain elusive due to the complexity of the involved (extra)cellular structures, such as the glycocalyx. We used nano-bio-interfaces reproducing the complex nanotopographical features of the extracellular matrix to analyse the glycocalyx impact on PC12 cell mechanosensing at the nanoscale (e.g., by force spectroscopy with functionalised probes). Our data demonstrates that the glycocalyx configuration affects spatio-temporal nanotopography-sensitive mechanotransductive events at the cell/microenvironment interface. Opposing effects of major glycocalyx removal were observed, when comparing flat and specific nanotopographical conditions. The excessive retrograde actin flow speed and force loading are strongly reduced on certain nanotopog- raphies upon strong reduction of the native glycocalyx, while on the flat substrate we observe the opposite trend. Our results highlight the importance of the glycocalyx configuration in a molecular clutch force loading-dependent cellular mechanism for mechanosensing of microenvironmental nanotopographical features.

The glycocalyx affects the mechanotransductive perception of the topographical microenvironment / M. Chighizola, T. Dini, S. Marcotti, M. D’Urso, C. Piazzoni, F. Borghi, A. Previdi, L. Ceriani, C. Folliero, B. Stramer, C. Lenardi, P. Milani, A. Podestà, C. Schulte. - In: JOURNAL OF NANOBIOTECHNOLOGY. - ISSN 1477-3155. - 20:1(2022), pp. 418.1-418.20. [10.1186/s12951-022-01585-5]

The glycocalyx affects the mechanotransductive perception of the topographical microenvironment

M. Chighizola
Primo
;
C. Piazzoni;F. Borghi;A. Previdi;C. Lenardi;P. Milani;A. Podestà
Penultimo
;
2022

Abstract

The cell/microenvironment interface is the starting point of integrin-mediated mechanotransduction, but many details of mechanotransductive signal integration remain elusive due to the complexity of the involved (extra)cellular structures, such as the glycocalyx. We used nano-bio-interfaces reproducing the complex nanotopographical features of the extracellular matrix to analyse the glycocalyx impact on PC12 cell mechanosensing at the nanoscale (e.g., by force spectroscopy with functionalised probes). Our data demonstrates that the glycocalyx configuration affects spatio-temporal nanotopography-sensitive mechanotransductive events at the cell/microenvironment interface. Opposing effects of major glycocalyx removal were observed, when comparing flat and specific nanotopographical conditions. The excessive retrograde actin flow speed and force loading are strongly reduced on certain nanotopog- raphies upon strong reduction of the native glycocalyx, while on the flat substrate we observe the opposite trend. Our results highlight the importance of the glycocalyx configuration in a molecular clutch force loading-dependent cellular mechanism for mechanosensing of microenvironmental nanotopographical features.
Adhesion force spectroscopy; Atomic force microscopy; Colloidal probes; Focal adhesion; Force loading; Glycocalyx; Integrin adhesion complexes; Mechanotransduction; Molecular clutch; Nanostructured cell microenvironment; Nanotopography
Settore FIS/03 - Fisica della Materia
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
   Biomechanics in health and disease: advanced physical tools for innovative early diagnosis (Phys2BioMed)
   Phys2BioMed
   EUROPEAN COMMISSION
   H2020
   812772

   Novel precision technological platforms to promote non-invasive early diagnosis, eradication and prevention of cancer relapse: proof of concept in the bladder carcinoma (EDIT)
   EDIT
   EUROPEAN COMMISSION
   H2020
   801126
2022
19-set-2022
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/938184
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