Anthropogenic organophosphorus compounds (AOPCs), such as phosphotriesters, areused extensively as plasticizers,flame retardants, nerve agents, and pesticides. To date,only a handful of soil bacteria bearing a phosphotriesterase (PTE), the key enzyme inthe AOPC degradation pathway, have been identified. Therefore, the extent to whichbacteria are capable of utilizing AOPCs as a phosphorus source, and how widespreadthis adaptation may be, remains unclear. Marine environments with phosphorus limita-tion and increasing levels of pollution by AOPCs may drive the emergence of PTEactivity. Here, we report the utilization of diverse AOPCs by four model marine bacte-ria and 17 bacterial isolates from the Mediterranean Sea and the Red Sea. To unravelthe details of AOPC utilization, two PTEs from marine bacteria were isolated and char-acterized, with one of the enzymes belonging to a protein family that, to our know-ledge, has never before been associated with PTE activity. When expressed inEscherichia coliwith a phosphodiesterase, a PTE isolated from a marine bacteriumenabled growth on a pesticide analog as the sole phosphorus source. Utilization ofAOPCs may provide bacteria a source of phosphorus in depleted environments andoffers a prospect for the bioremediation of a pervasive class of anthropogenic pollutants.

Utilization of diverse organophosphorus pollutants by marine bacteria / D. Despotovi('(c)), E. Aharon, O. Trofimyuk, A. Dubovetskyi, K. Phaneendra Cherukuri, Y. Ashani, O. Eliason, M. Sperfeld, H. Leader, A. Castelli, L. Fumagalli, A. Savidor, Y. Levin, L.M. Longo, E. Segev, D.S. Tawfik. - In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. - ISSN 0027-8424. - 119:32(2022 Aug 09), pp. e2203604119.1-e2203604119.8. [10.1073/pnas.2203604119]

Utilization of diverse organophosphorus pollutants by marine bacteria

L. Fumagalli;
2022-08-09

Abstract

Anthropogenic organophosphorus compounds (AOPCs), such as phosphotriesters, areused extensively as plasticizers,flame retardants, nerve agents, and pesticides. To date,only a handful of soil bacteria bearing a phosphotriesterase (PTE), the key enzyme inthe AOPC degradation pathway, have been identified. Therefore, the extent to whichbacteria are capable of utilizing AOPCs as a phosphorus source, and how widespreadthis adaptation may be, remains unclear. Marine environments with phosphorus limita-tion and increasing levels of pollution by AOPCs may drive the emergence of PTEactivity. Here, we report the utilization of diverse AOPCs by four model marine bacte-ria and 17 bacterial isolates from the Mediterranean Sea and the Red Sea. To unravelthe details of AOPC utilization, two PTEs from marine bacteria were isolated and char-acterized, with one of the enzymes belonging to a protein family that, to our know-ledge, has never before been associated with PTE activity. When expressed inEscherichia coliwith a phosphodiesterase, a PTE isolated from a marine bacteriumenabled growth on a pesticide analog as the sole phosphorus source. Utilization ofAOPCs may provide bacteria a source of phosphorus in depleted environments andoffers a prospect for the bioremediation of a pervasive class of anthropogenic pollutants.
anthropogenic organophosphorus compounds; bioremediation; marine bacteria; phosphotriesterases; Biodegradation, Environmental; Escherichia coli; Indian Ocean; Mediterranean Sea; Phosphorus; Seawater; Aquatic Organisms; Bacteria; Environmental Pollutants; Organophosphorus Compounds; Phosphoric Triester Hydrolases;
Settore CHIM/08 - Chimica Farmaceutica
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/937215
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