Apatite/Tricalcium phosphate composite cement composed with polyacrylic acid (PAA-apatite/TCP) has previously been reported to be a great promise for orthopedic applications. However, it was investigated that PAA could inhibit apatite phase conversion and result in poor bioactivity. To improve those limitations, PAA-apatite/ TCP cement modified with the bioactive glass nanoparticle (0-1.5 wt%) was investigated for physical properties, phase formation after setting and bioactivity. In addition, human Adipose-derived Stem/Stromal cells (hASCs), which were isolated from human adipose tissue and represent a widely implemented cell type for tissue engineering applications, were selected to determine their biological response to the bioactive glass modified PAAapatite/TCP cement. The results indicated that the PAA-apatite/TCP cement added with bioactive glass cement decreased setting time and enhanced apatite phase transformation and bioactivity, as compared with the control cement. For in vitro testing, hASCs viability, proliferation, and differentiation potential were assessed under two cell culture conditions. In details, cells were either cultured with cement-preconditioned medium (indirect setting) or with cement (direct setting). The indirect culture presented a lower proliferation rate as compared with the control medium. In contrast, the result of direct culture indicated a higher proliferation rate on both control cement and 1 wt% bioactive glass incorporated cement, as compared with coverslip control. Moreover, the increase in Alkaline Phosphatase activity following osteogenic stimuli indicated that both cements supported osteogenic commitment. In conclusion, these in vitro data lay the basis for a deeper investigation of 1 wt% bioactive glass incorporated cement as a promising formula for clinical applications in bone engineering and orthopedics.
Nano-bioactive glass incorporated polymeric Apatite/Tricalcium phosphate cement composite supportsvproliferation and osteogenic differentiation of human adipose-derived stem/stromal cells / P. Thaitalay, C. Giannasi, S. Niada, O. Thongsri, R. Dangviriyakul, S. Srisuwan, S. Suksaweang, A.T.M. Brini, S. Tubsungnoen Rattanachana. - In: MATERIALS TODAY COMMUNICATIONS. - ISSN 2352-4928. - 31:(2022 Jun), pp. 103590.1-103590.12. [10.1016/j.mtcomm.2022.103590]
Nano-bioactive glass incorporated polymeric Apatite/Tricalcium phosphate cement composite supportsvproliferation and osteogenic differentiation of human adipose-derived stem/stromal cells
C. Giannasi;S. Niada;A.T.M. BriniUltimo
;
2022
Abstract
Apatite/Tricalcium phosphate composite cement composed with polyacrylic acid (PAA-apatite/TCP) has previously been reported to be a great promise for orthopedic applications. However, it was investigated that PAA could inhibit apatite phase conversion and result in poor bioactivity. To improve those limitations, PAA-apatite/ TCP cement modified with the bioactive glass nanoparticle (0-1.5 wt%) was investigated for physical properties, phase formation after setting and bioactivity. In addition, human Adipose-derived Stem/Stromal cells (hASCs), which were isolated from human adipose tissue and represent a widely implemented cell type for tissue engineering applications, were selected to determine their biological response to the bioactive glass modified PAAapatite/TCP cement. The results indicated that the PAA-apatite/TCP cement added with bioactive glass cement decreased setting time and enhanced apatite phase transformation and bioactivity, as compared with the control cement. For in vitro testing, hASCs viability, proliferation, and differentiation potential were assessed under two cell culture conditions. In details, cells were either cultured with cement-preconditioned medium (indirect setting) or with cement (direct setting). The indirect culture presented a lower proliferation rate as compared with the control medium. In contrast, the result of direct culture indicated a higher proliferation rate on both control cement and 1 wt% bioactive glass incorporated cement, as compared with coverslip control. Moreover, the increase in Alkaline Phosphatase activity following osteogenic stimuli indicated that both cements supported osteogenic commitment. In conclusion, these in vitro data lay the basis for a deeper investigation of 1 wt% bioactive glass incorporated cement as a promising formula for clinical applications in bone engineering and orthopedics.File | Dimensione | Formato | |
---|---|---|---|
Paritat Thaitalay et al. 2022_Materials Today Communications.pdf
accesso riservato
Tipologia:
Publisher's version/PDF
Dimensione
8.57 MB
Formato
Adobe PDF
|
8.57 MB | Adobe PDF | Visualizza/Apri Richiedi una copia |
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.