Phosphorous (P) is an essential macroelement for plant development. Despite P is a major element in soil, it is often present in forms that are not available for plant uptake. Growing attention is directed towards P-solubilising microbes able to solubilize insoluble forms of P, making them available for plants. The content of P in soils is influenced by agronomic practices, but despite its importance in plant nutrition, the knowledge associated with this element is still scarce and there is a gap in the knowledge related to the mechanisms that affect P content. The present work aims at exploring the bacterial communities associated with the rhizosphere of rice cultivated in soils that underwent different agronomic practices, to detect bacteria that show plant growth-promoting activities (PGPA), in particular concerning P solubilisation. Rhizosphere and root samples of rice plants were collected from two paddies, one treated with phosphate and the other with a cover crop. Bacteria were isolated from different compartments of the rhizosphere: rhizosphere soil, root epiphytic biofilm (rhizoplane) and root endosphere. A total of 558 isolates (254 strains from the rhizosphere soil, 100 from the rhizoplane and 204 from the root endosphere) underwent de-replication at the strain level according to BOX-PCR analysis performed on capillary electrophoresis. Up to 5 candidates per each BOX profile variant were further screened for PGPA like hydrolysation of inorganic and organic P, production of indole-3-acetic acid (IAA) and siderophores, fixation of atmospheric nitrogen, and ACC deaminase activity. Of the 231 isolates tested, 25.1% showed tricalcium phosphate-solubilising abilities, among them 34 strains were isolated from the root endosphere and 24 from the rhizosphere. Moreover, 9 out of 11 rhizosphere isolates produced IAA. The promising isolates will be tested in vivo for their promotion of rice seed germination in growth pouches and, further, in soil pot experiments. The output of this project will help to understand the role of P-solubilizing bacteria in P rice nutrition and develop biofertilizers able to enhance the uptake of soil endogenous P.

Phosporous in rice paddy field, balance between productivity and environment in view of new agronomic practices / F. Boiocchi, S. Zecchin, M. Colombo, S. Foiani, C. Valli, M. Bertolini, M. Martin, L. Celi, M. Romani, F. Miniotti Eleonora, L. Cavalca. ((Intervento presentato al convegno World Microbe Forum tenutosi a Online nel 2021.

Phosporous in rice paddy field, balance between productivity and environment in view of new agronomic practices

F. Boiocchi
Primo
;
S. Zecchin
Secondo
;
M. Colombo;S. Foiani;C. Valli;M. Bertolini;L. Cavalca
Ultimo
2021

Abstract

Phosphorous (P) is an essential macroelement for plant development. Despite P is a major element in soil, it is often present in forms that are not available for plant uptake. Growing attention is directed towards P-solubilising microbes able to solubilize insoluble forms of P, making them available for plants. The content of P in soils is influenced by agronomic practices, but despite its importance in plant nutrition, the knowledge associated with this element is still scarce and there is a gap in the knowledge related to the mechanisms that affect P content. The present work aims at exploring the bacterial communities associated with the rhizosphere of rice cultivated in soils that underwent different agronomic practices, to detect bacteria that show plant growth-promoting activities (PGPA), in particular concerning P solubilisation. Rhizosphere and root samples of rice plants were collected from two paddies, one treated with phosphate and the other with a cover crop. Bacteria were isolated from different compartments of the rhizosphere: rhizosphere soil, root epiphytic biofilm (rhizoplane) and root endosphere. A total of 558 isolates (254 strains from the rhizosphere soil, 100 from the rhizoplane and 204 from the root endosphere) underwent de-replication at the strain level according to BOX-PCR analysis performed on capillary electrophoresis. Up to 5 candidates per each BOX profile variant were further screened for PGPA like hydrolysation of inorganic and organic P, production of indole-3-acetic acid (IAA) and siderophores, fixation of atmospheric nitrogen, and ACC deaminase activity. Of the 231 isolates tested, 25.1% showed tricalcium phosphate-solubilising abilities, among them 34 strains were isolated from the root endosphere and 24 from the rhizosphere. Moreover, 9 out of 11 rhizosphere isolates produced IAA. The promising isolates will be tested in vivo for their promotion of rice seed germination in growth pouches and, further, in soil pot experiments. The output of this project will help to understand the role of P-solubilizing bacteria in P rice nutrition and develop biofertilizers able to enhance the uptake of soil endogenous P.
20-giu-2021
Settore AGR/16 - Microbiologia Agraria
Federation of European Microbiological Societies (FEMS)
American Societies for Microbiology (ASM)
Phosporous in rice paddy field, balance between productivity and environment in view of new agronomic practices / F. Boiocchi, S. Zecchin, M. Colombo, S. Foiani, C. Valli, M. Bertolini, M. Martin, L. Celi, M. Romani, F. Miniotti Eleonora, L. Cavalca. ((Intervento presentato al convegno World Microbe Forum tenutosi a Online nel 2021.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/925678
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