The use of sulfur (S) stable isotopes to study S metabolism in plants is still limited by the relatively small number of studies. It is generally accepted that less S stable isotope discrimination occurs during sulfate (SO42–) uptake. However, S metabolism and allocation are expected to produce separations of S stable isotopes among the different plant S pools and organs. In this study, we measured the S isotope composition of the main S pools of rice plants grown under different SO42– availabilities in appropriate closed and open hydroponic-plant systems. The main results indicate that fractionation against 34S occurred during SO42– uptake. Fractionation was dependent on the amount of residual SO42– in the solution, showing a biphasic behavior related to the relative expression of two SO42– transporter genes (OsSULTR1;1 and OsSULTR1;2) in the roots. S isotope separations among S pools and organs were also observed as the result of substantial S isotope fractionations and mixing effects occurring during SO42– assimilation and plant S partitioning. Since the S stable isotope separations conserve the memory of the physiological and metabolic activities that determined them, we here underline the potential of the 32S/34S analysis for the detailed characterization of the metabolic and molecular processes involved in plant S nutrition and homeostasis.
Sulfur stable isotope discrimination in rice: a sulfur isotope mass balance study / V. Cavallaro, M. Maghrebi, M. Caschetto, G.A. Sacchi, F.F. Nocito. - In: FRONTIERS IN PLANT SCIENCE. - ISSN 1664-462X. - 13:(2022 Mar 10), pp. 837517.1-837517.11. [10.3389/fpls.2022.837517]
Sulfur stable isotope discrimination in rice: a sulfur isotope mass balance study
V. CavallaroPrimo
;M. MaghrebiSecondo
;M. Caschetto;G.A. SacchiPenultimo
;F.F. Nocito
Ultimo
2022
Abstract
The use of sulfur (S) stable isotopes to study S metabolism in plants is still limited by the relatively small number of studies. It is generally accepted that less S stable isotope discrimination occurs during sulfate (SO42–) uptake. However, S metabolism and allocation are expected to produce separations of S stable isotopes among the different plant S pools and organs. In this study, we measured the S isotope composition of the main S pools of rice plants grown under different SO42– availabilities in appropriate closed and open hydroponic-plant systems. The main results indicate that fractionation against 34S occurred during SO42– uptake. Fractionation was dependent on the amount of residual SO42– in the solution, showing a biphasic behavior related to the relative expression of two SO42– transporter genes (OsSULTR1;1 and OsSULTR1;2) in the roots. S isotope separations among S pools and organs were also observed as the result of substantial S isotope fractionations and mixing effects occurring during SO42– assimilation and plant S partitioning. Since the S stable isotope separations conserve the memory of the physiological and metabolic activities that determined them, we here underline the potential of the 32S/34S analysis for the detailed characterization of the metabolic and molecular processes involved in plant S nutrition and homeostasis.File | Dimensione | Formato | |
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