Transient nuclear envelope ruptures during interphase (NERDI) occur due to cytoskeletal compressive forces at sites of weakened lamina, and delayed NERDI repair results in genomic instability. Nuclear envelope (NE) sealing is completed by endosomal sorting complex required for transport (ESCRT) machinery. A key unanswered question is how local compressive forces are counteracted to allow efficient membrane resealing. Here, we identify the ESCRT-associated protein BROX as a crucial factor required to accelerate repair of the NE. Critically, BROX binds Nesprin-2G, a component of the linker of nucleoskeleton and cytoskeleton complex (LINC). This interaction promotes Nesprin-2G ubiquitination and facilitates the relaxation of mechanical stress imposed by compressive actin fibers at the rupture site. Thus, BROX rebalances excessive cytoskeletal forces in cells experiencing NE instability to promote effective NERDI repair. Our results demonstrate that BROX coordinates mechanoregulation with membrane remodeling to ensure the maintenance of nuclear-cytoplasmic compartmentalization and genomic stability.

The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair / S.S. Wallis, L.N. Ventimiglia, E. Otigbah, E. Infante, M.A. Cuesta-Geijo, G.R. Kidiyoor, M.A. Carbajal, R.A. Fleck, M. Foiani, S. Garcia-Manyes, J. Martin-Serrano, M. Agromayor. - In: DEVELOPMENTAL CELL. - ISSN 1534-5807. - 56:23(2021 Dec 06), pp. 3192-3202.e8. [10.1016/j.devcel.2021.10.022]

The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair

M. Foiani;
2021

Abstract

Transient nuclear envelope ruptures during interphase (NERDI) occur due to cytoskeletal compressive forces at sites of weakened lamina, and delayed NERDI repair results in genomic instability. Nuclear envelope (NE) sealing is completed by endosomal sorting complex required for transport (ESCRT) machinery. A key unanswered question is how local compressive forces are counteracted to allow efficient membrane resealing. Here, we identify the ESCRT-associated protein BROX as a crucial factor required to accelerate repair of the NE. Critically, BROX binds Nesprin-2G, a component of the linker of nucleoskeleton and cytoskeleton complex (LINC). This interaction promotes Nesprin-2G ubiquitination and facilitates the relaxation of mechanical stress imposed by compressive actin fibers at the rupture site. Thus, BROX rebalances excessive cytoskeletal forces in cells experiencing NE instability to promote effective NERDI repair. Our results demonstrate that BROX coordinates mechanoregulation with membrane remodeling to ensure the maintenance of nuclear-cytoplasmic compartmentalization and genomic stability.
ESCRT; LINC complex; mechanosensing; membrane repair; nuclear envelope; Actins; Cell Movement; Cell Nucleus; Cytoskeleton; Endosomal Sorting Complexes Required for Transport; HeLa Cells; Humans; Mechanical Phenomena; Microfilament Proteins; Nerve Tissue Proteins; Nuclear Envelope
Settore BIO/11 - Biologia Molecolare
   Mechanical control of biological function
   MECHANO-CONTROL
   European Commission
   Horizon 2020 Framework Programme
   731957

   High-resolution, large scanning atomic force microscope (AFM) for capturing cellular processes in action
   UK Research and Innovation
   EPSRC
   EP/M022536/1

   Understanding the role of the ESCRT-associated protein MITD1 in linking cytoskeleton and membrane remodelling
   UK Research and Innovation
   BBSRC
   BB/N000501/1
6-dic-2021
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/915371
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