The alkenylbenzene estragole (systematic name, 1-allyl-4-methoxybenzene) is a natural component of essential oils from various spices and herbs, including fennel, and it is used as a food and beverage flavouring agent. Estragole has been reported to be hepatocarcinogenic at high doses in rodents. However, in a previous in vitro study, we found that estragole did not exhibit cytotoxic effects after 4 hours of exposure, nor did it induce DNA damage or apoptosis in human HepG2 hepatoblastoma cells. As fennel tea is widely used for symptomatic treatment of spasmodic gastrointestinal conditions in infants, we aimed at further assessing its safety in a different experimental setting. We thus searched for possible cytogenetic effects and interference with cell-cycle progression in the same human hepatoblastoma cell line. Estragole did not show any clastogenic/aneugenic activities in the cytokinesis-block micronucleus assay, and no effects on cell-cycle checkpoints were observed.
In vitro testing of estragole in hepg2 cells: Cytokinesis-block micronucleus assay and cell-cycle analysis / S. Vannini, R. Pagiotti, M. Acito, S. Levorato, L. Dominici, C. Fatigoni, V. Gianfredi, M. Moretti, M. Villarini. - In: ACTA ALIMENTARIA. - ISSN 0139-3006. - 47:2(2018 May 23), pp. 195-202. [10.1556/066.2018.47.2.8]
In vitro testing of estragole in hepg2 cells: Cytokinesis-block micronucleus assay and cell-cycle analysis
V. Gianfredi;
2018
Abstract
The alkenylbenzene estragole (systematic name, 1-allyl-4-methoxybenzene) is a natural component of essential oils from various spices and herbs, including fennel, and it is used as a food and beverage flavouring agent. Estragole has been reported to be hepatocarcinogenic at high doses in rodents. However, in a previous in vitro study, we found that estragole did not exhibit cytotoxic effects after 4 hours of exposure, nor did it induce DNA damage or apoptosis in human HepG2 hepatoblastoma cells. As fennel tea is widely used for symptomatic treatment of spasmodic gastrointestinal conditions in infants, we aimed at further assessing its safety in a different experimental setting. We thus searched for possible cytogenetic effects and interference with cell-cycle progression in the same human hepatoblastoma cell line. Estragole did not show any clastogenic/aneugenic activities in the cytokinesis-block micronucleus assay, and no effects on cell-cycle checkpoints were observed.File | Dimensione | Formato | |
---|---|---|---|
066.2018.47.2.8.pdf
accesso aperto
Tipologia:
Publisher's version/PDF
Dimensione
115.82 kB
Formato
Adobe PDF
|
115.82 kB | Adobe PDF | Visualizza/Apri |
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.