The fabrication of in vitro neuronal cell networks where cells are chemically or electrically connected to form functional circuits with useful properties is of great interest. Standard cell culture substrates provide ensembles of cells that scarcely reproduce physiological structures since their spatial organization and connectivity cannot be controlled. Supersonic Cluster Beam Deposition (SCBD) has been used as an effective additive method for the large-scale fabrication of interfaces with extracellular matrix-mimicking surface nanotopography and reproducible morphological properties for cell culture. Due to the high collimation of SCBD, it is possible to exploit stencil masks for the fabrication of patterned films and reproduce features as small as tens of micrometers. Here, we present a protocol to fabricate micropatterned cell culture substrates based on the deposition of nanostructured cluster-assembled zirconia films by stencil-assisted SCBD. The effectiveness of this approach is demonstrated by the fabrication of micrometric patterns able to confine primary astrocytes. Calcium waves propagating in the astrocyte networks are shown.

Micropatterning of Substrates for the Culture of Cell Networks by Stencil-Assisted Additive Nanofabrication / A. Previdi, C. Piazzoni, F. Borghi, C. Schulte, L. Lorenzelli, F. Giacomozzi, A. Bucciarelli, A. Malgaroli, J. Lamanna, A. Moro, G. Racchetti, P. Alessandro, C. Lenardi, P. Milani. - In: MICROMACHINES. - ISSN 2072-666X. - 12:1(2021 Jan 18), pp. 94.1-94.18. [10.3390/mi12010094]

Micropatterning of Substrates for the Culture of Cell Networks by Stencil-Assisted Additive Nanofabrication

A. Previdi
Writing – Original Draft Preparation
;
C. Piazzoni
Membro del Collaboration Group
;
F. Borghi
Methodology
;
C. Schulte
Methodology
;
A.S. Moro
Investigation
;
P. Alessandro
Methodology
;
C. Lenardi
Penultimo
Methodology
;
P. Milani
Ultimo
Conceptualization
2021-01-18

Abstract

The fabrication of in vitro neuronal cell networks where cells are chemically or electrically connected to form functional circuits with useful properties is of great interest. Standard cell culture substrates provide ensembles of cells that scarcely reproduce physiological structures since their spatial organization and connectivity cannot be controlled. Supersonic Cluster Beam Deposition (SCBD) has been used as an effective additive method for the large-scale fabrication of interfaces with extracellular matrix-mimicking surface nanotopography and reproducible morphological properties for cell culture. Due to the high collimation of SCBD, it is possible to exploit stencil masks for the fabrication of patterned films and reproduce features as small as tens of micrometers. Here, we present a protocol to fabricate micropatterned cell culture substrates based on the deposition of nanostructured cluster-assembled zirconia films by stencil-assisted SCBD. The effectiveness of this approach is demonstrated by the fabrication of micrometric patterns able to confine primary astrocytes. Calcium waves propagating in the astrocyte networks are shown.
astrocytes; cell confinement; micropatterns; nanofabrication; nanostructured zirconia; primary cell networks
Settore FIS/03 - Fisica della Materia
Article (author)
File in questo prodotto:
File Dimensione Formato  
micromachines-12-00094.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 3.8 MB
Formato Adobe PDF
3.8 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/907906
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 0
  • ???jsp.display-item.citation.isi??? 0
social impact